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SELECTBIO Conferences BioMEMS, Microfluidics & Biofabrication: Technologies and Applications
Conferences  \  BioEngineering 2017: BioMEMS, 3D-BioPrinting & Synthetic Biology  \  BioMEMS, Microfluidics & Biofabrication: Technologies and Applications  \  Agenda  \  Yagmur Yildizhan
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Yagmur Yildizhan's Biography


Yagmur Yildizhan, Researcher, Biosensors Group, KU Leuven

Yagmur Yildizhan received her B.Sc. degree in Physics Department at Bilkent University in 2016 and her M.Sc. degree in Mechatronics Engineering Program at Sabanci University in 2018 where she conducted her research on dielectrophoretic characterization and separation of cells. Currently, she continues her research in the Biosensors group at KU Leuven, Belgium (headed by prof. Jeroen Lammertyn) as Marie Curie fellow within the framework of H2020 ITN project AiPBAND (“An integrated platform for developing brain cancer diagnostic techniques”). Her research focuses on developing fiber optic surface plasmon resonance and digital ELISA technology platforms for ultrasensitive detection of blood-based tumor biomarkers, particularly extracellular vesicles.

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Characterization and Separation of U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Electrode Dielectrophoresis

Friday, 17 March 2017 at 17:00

Add to Calendar ▼2017-03-17 17:00:002017-03-17 18:00:00Europe/LondonCharacterization and Separation of U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Electrode DielectrophoresisSELECTBIOenquiries@selectbiosciences.com

Tumor-associated macrophages (TAM), one of the key players in tumor microenvironment, are involved in tumor development and progression in many cancers. Here, we will present carbon electrode dielectrophoresis (carbon-DEP) as a characterization tool to identify and separate U937 monocytes and U937-differentiated macrophages. First, we will differentiate monocytes to obtain macrophages. Next, we will apply carbon-DEP to determine specific crossover frequencies as signatures. Since DEP does not require any pre-labeling for the cells, it will allow direct characterization of cells based on their physical intrinsic properties without altering their genetic and phenotypic properties. Finally, we will verify our results using traditional assays. When we obtain their dielectrophoretic signatures, we will isolate and enrich them for high-throughput biochemical analysis.

Add to Calendar ▼2017-03-16 00:00:002017-03-17 00:00:00Europe/LondonBioMEMS, Microfluidics and Biofabrication: Technologies and ApplicationsSELECTBIOenquiries@selectbiosciences.com


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