Conferences \ BioEngineering 2017: BioMEMS, 3D-BioPrinting & Synthetic Biology \ 3D-Bioprinting, Tissue Engineering and Synthetic Biology \ Agenda \ Elena Bulanova |
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Scalable Biofabrication and Systematic Characterization of Tissue Spheroids for Directed Tissue Self-Assembly Using Acoustic WavesFriday, 17 March 2017 at 12:00 Add to Calendar ▼2017-03-17 12:00:002017-03-17 13:00:00Europe/LondonScalable Biofabrication and Systematic Characterization of Tissue Spheroids for Directed Tissue Self-Assembly Using Acoustic WavesSELECTBIOenquiries@selectbiosciences.com Tissue spheroids are gaining extensively their place in biofabrication as building blocks. We suggested a straightforward procedure for biofabrication and initial characterization of tissue spheroids with optimal controllable parameters prepared from different cell types employing non-adhesive technology. Applying different immortalized and primary cells we have demonstrated the reproducibility and scalability of spheroid generation, the strong dependency of ultimate spheroid diameter on initial cell seeding density and cell type. Additionally, the spheroids viability was shown to be governed by cell derivation. In our study we suggest a decision procedure to apply for any cell type one starts to work with to prepare a new type of tissue spheroids with predictable controllable optimal features suitable for high quality standards in biofabrication and drug discovery. |