Shayne Harrel,
Chief Scientist-Americas,
Izon Science US LTD
Shayne Harrel is the Chief Scientist of the Americas for Izon Science. Before joining Izon, he worked at Olympus Corporation of the Americas as a Senior Applications Scientist specializing in x-ray fluorescence spectroscopy (XRF). Shayne also worked as a chemist for the USFDA where he focused on radio and analytical chemistry, measurement uncertainty analysis and regulatory science.
Shayne holds a PhD in physical chemistry from Yale University. During his graduate career, he helped pioneer the development and application of Terahertz emission spectroscopy (TES) to charge transfer systems, semiconductor and magnetic materials.
Isolation and Characterization of Extracellular Vesicles; An Integrated, High-Precision and Standardizable Approach
Monday, 17 February 2020 at 16:00
Add to Calendar ▼2020-02-17 16:00:002020-02-17 17:00:00Europe/LondonIsolation and Characterization of Extracellular Vesicles; An Integrated, High-Precision and Standardizable ApproachCirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Tremendous interest exists towards the development and implementation of extracellular vesicles (EVs) in bio-fluids as rich sources of diagnostic and prognostic biomarkers. EVs derived from bio-fluids exhibit extensive heterogeneity with regards to particle size, concentration, membrane composition and cargo resulting in unique challenges in terms of EVs isolation and characterization. We present an approach to purify EVs from complex bio-fluids using Izon’s qEV line of size exclusion chromatography (SEC) based columns, resulting in a highly pure and unperturbed sample. Reproducibility and standardization is enhanced by incorporating Izon’s Automated Fraction Collector (AFC) into the isolation. Tunable Resistive Pulse Sensing (TRPS) is used to characterize the biophysical properties (size, concentration and Zeta Potential) of purified EVs. TRPS measurements are conducted at the single particle level and results are compared against a known standard, ensuring the accuracy, repeatability and traceability of the data.
Add to Calendar ▼2020-02-17 00:00:002020-02-18 00:00:00Europe/LondonCirculating Biomarkers World Congress 2020Circulating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2020-02-17 16:00:002020-02-17 17:00:00Europe/LondonIsolation and Characterization of Extracellular Vesicles; An Integrated, High-Precision and Standardizable ApproachCirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
