Conferences \ Extracellular Vesicles 2022: Biology, Disease & Medicine \ Agenda \ Daniel Chiu |
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Digital Flow Cytometry For Single-EV AnalysisTuesday, 13 September 2022 at 12:00 Add to Calendar ▼2022-09-13 16:30:002022-09-13 17:30:00Europe/LondonNew Fluorescent Reagents to Enable Highly Multiplexed Single-Cell Measurements and Biological AssaysExtracellular Vesicles 2022: Biology, Disease and Medicine in SeattleSeattleSELECTBIOenquiries@selectbiosciences.com We have developed a multi-parametric high-throughput flow-based method for the analysis of individual extracelluar vesicles (EVs), which are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. Yet, current approaches to the study of EVs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EV subpopulations that may be present. Digital flow cytometry provides single-fluorophore sensitivity to enable phenotyping single EVs with unprecedented precision and sensitivity. New Fluorescent Reagents to Enable Highly Multiplexed Single-Cell Measurements and Biological AssaysTuesday, 13 September 2022 at 16:30 Add to Calendar ▼2022-09-13 16:30:002022-09-13 17:30:00Europe/LondonNew Fluorescent Reagents to Enable Highly Multiplexed Single-Cell Measurements and Biological AssaysExtracellular Vesicles 2022: Biology, Disease and Medicine in SeattleSeattleSELECTBIOenquiries@selectbiosciences.com Fluorescence based techniques have become an indispensible tool kit in both basic cellular studies and in vitro diagnostics. However, the intrinsic limitations of conventional dyes, such as short Stoke’s shift and low absorptivity, have posed difficulties for advancing highly multiplexed assays. We have developed a new class of fluorescent probes called Pdots, and this talk will highlight their development to enable high multiplex single-cell analysis and biological assays. |