Maria Giraldez,
Researcher,
University of Michigan
Maria D. Giraldez obtained her MD-PhD at the University of Barcelona (Spain) where she focused her research on improving the strategies for diagnosis and prevention of digestive malignancies, especially hereditary and familial forms of colorectal cancer, using genetic approaches. After defending her doctoral dissertation, she joined Tewari lab (initially based on Fred Hutchinson Cancer Research Center, Seattle, WA, and currently at the University of Michigan, Ann Arbor, MI) to get involved in the use of cutting-edge technologies to identify nucleic acid-based circulating biomarkers in human cancer. She is currently evaluating the potential utility of different types of cell-free nucleic acids as non-invasive biomarkers in a variety of clinical settings. Her final goal is translating laboratory-based discoveries into effective diagnostic/predictive tools suitable for clinical use.
Accuracy, Reproducibility and Consequences of Bias in Quantitative Small RNA-seq For Circulating Cell-free RNA Profiling
Thursday, 25 October 2018 at 09:30
Add to Calendar ▼2018-10-25 09:30:002018-10-25 10:30:00Europe/LondonAccuracy, Reproducibility and Consequences of Bias in Quantitative Small RNA-seq For Circulating Cell-free RNA ProfilingExosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Small RNA-seq is increasingly being used for profiling of small RNAs, including cell-free RNA such as microRNAs present in plasma and other biofluids. In practice, small RNAseq can involve many different methods for library preparation, with distinct protocols and technical variations that could cause substantial differences in results, yet these have not been fully and systematically studied. We report here the results of a study using common references (synthetic RNA pools of defined composition, as well as plasma-derived RNA) to evaluate the accuracy, reproducibility and bias of small RNA-seq library preparation for five distinct protocols and across nine different laboratories. We observed protocol-specific and sequence-specific bias, which was ameliorated using adapters with randomized end-nucleotides for ligation. Despite this technical bias, relative quantification using small RNA-seq was remarkably accurate and reproducible, even across multiple laboratories using different methods. These results provide a foundation for reproducible, cross-laboratory small RNAseq studies of cell-free, circulating RNA in a variety of clinical contexts.
Add to Calendar ▼2018-10-24 00:00:002018-10-26 00:00:00Europe/LondonExosomes and Liquid Biopsies Europe 2018Exosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2018-10-25 09:30:002018-10-25 10:30:00Europe/LondonAccuracy, Reproducibility and Consequences of Bias in Quantitative Small RNA-seq For Circulating Cell-free RNA ProfilingExosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
