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Quantitative Imaging and Phenotyping of EVs with 20-nm Resolution

Thursday, 31 October 2019 at 12:00

Add to Calendar ▼2019-10-31 12:00:002019-10-31 13:00:00Europe/LondonQuantitative Imaging and Phenotyping of EVs with 20-nm ResolutionCirculating Biomarkers, Exosomes and Liquid Biopsy Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

Complex extracellular vesicle (EV) phenotyping is a major technical challenge that hinders clinical translation. The evaluation of EV biomarkers is further challenged by the small size of these effectors of cell-to-cell communication and the limited sensitivity and resolution of most available techniques. Single-molecule detection of fluorescently labeled EV components (such as membrane, proteins, RNA/DNA) is a robust approach to accurately and quantitatively analyze the molecular composition of individual EVs from mixed EV populations. ONI has developed the Nanoimager, the only benchtop single-molecule microscope that integrates the most advanced single-particle detection and analysis methods of EVs in tissues, in solution or in live cells. In this presentation we show how the Nanoimager allows biomarker identification, sizing, counting and detection of cell interactions of single EVs down to 20 nm resolution. This technology consolidates single-molecule imaging methods as a sensitive, simple, robust and scalable approach to characterize EV properties from complex molecular environment.