Shlomit Kenigsberg,
Senior Researcher,
Create Fertility Centre
I am currently a senior researcher and lead for the Ovarian Biology Research group at CReATe Fertility Centre. My research is primarily focused on the isolation and characterizing of exosomes that are secreted from granulosa cells (GLCs) into follicular fluid (FF), and to study their unique RNAs (miRNome) and protein (Proteome) content. We plan to look for differences in exosomes content between different fertility-related patient groups. My long term goals are to 1) provide serum-based biomarkers to help asses a patient’s fertility potential and diagnosis, and 2) to discover potential FF biomarkers to assess egg quality and potential to create a healthy embryo.
Isolation, Visualization and Characterization of Exosomes Found in Human Follicular Fluid: A Potentially Important Mechanism for Intra-Follicular Cell-Cell Communication
Tuesday, 28 October 2014 at 16:00
Add to Calendar ▼2014-10-28 16:00:002014-10-28 17:00:00Europe/LondonIsolation, Visualization and Characterization of Exosomes Found in Human Follicular Fluid: A Potentially Important Mechanism for Intra-Follicular Cell-Cell CommunicationSELECTBIOenquiries@selectbiosciences.com
Follicular fluid (FF) provides part of the microenvironment that regulates oocyte development and thus, may play a critical role in oocyte fertilization and embryo development. Intercellular signaling between granulosa cells (GLC), cumulus cells (CM), and the oocyte is required for proper folliculogenesis, ovulation, and hormonal secretion. These signals can be mediated by small membranous vesicles called exosomes that are 50-150nm in size and contain a unique repertoire of proteins and microRNAs (miRNAs). The recently discovered tissue and fluid distributions of exosomes elude to their potential biological relevance. Evidence suggests their involvement in cell-cell signaling. Therefore, we hypothesize that exosomes mediate intercellular signaling within ovarian follicles. Our aim was to isolate and characterize both human FF containing exosomes and-exosomes secreted by granulosa cells in vitro, using a variety of biochemical and cell biology techniques. Body fluid extracellular particle isolation techniques, characterization and visualization, as well as their potential as biomarkers for oocyte quality, will be discussed.
Add to Calendar ▼2014-10-27 00:00:002014-10-28 00:00:00Europe/LondonBiofluid BiopsiesSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-10-28 16:00:002014-10-28 17:00:00Europe/LondonIsolation, Visualization and Characterization of Exosomes Found in Human Follicular Fluid: A Potentially Important Mechanism for Intra-Follicular Cell-Cell CommunicationSELECTBIOenquiries@selectbiosciences.com
