Gregory Cooksey,
Project Leader,
National Institute of Standards and Technology (NIST)
Dr. Cooksey is a Project Leader in the Microsystems and Nanotechnology Division at the National Institute of Standards and Technology (NIST). His lab develops microfluidic platforms with integrated optical structures to measure fluids, particles, and cells with high precision. His team’s optofluidic flow meter is the most accurate flow metrology platform in the world, and it has a response time on the order of 0.1 s at 1 nL/min. Dr. Cooksey also leads a microfluidic cytometry project that uses multiple interrogation regions to extract first ever per-object uncertainty quantification and measurement of dynamics in flow. This technology was awarded a Gold Medal and the 2023 Ron Brown Excellence in Innovation Award by the U.S. Department of Commerce.
A Microfluidic Serial Cytometer to Estimate Per-Cell Uncertainty and Single Object Kinetic Measurements
Tuesday, 19 November 2024 at 15:30
Add to Calendar ▼2024-11-19 15:30:002024-11-19 16:30:00Europe/LondonA Microfluidic Serial Cytometer to Estimate Per-Cell Uncertainty and Single Object Kinetic MeasurementsInnovations in Flow Cytometry and Extracellular Vesicles 2024 in Laguna Hills, CaliforniaLaguna Hills, CaliforniaSELECTBIOenquiries@selectbiosciences.com
NIST has developed a serial microfluidic cytometer that repeats the interrogation of objects at multiple points along a flow path, which enables direct assessment of uncertainty in cytometry measurements (DiSalvo et al., Lab Chip 2022). We have achieved per-particle measurement variation below 2 % at throughputs above 100 s-1 and detection limits and dynamic range comparable to conventional cytometers. This presentation will introduce various measurement capabilities novel to our serial cytometry, including 1) uncertainty quantification, 2) signals analysis that permit estimation of velocity, size, and shape of samples, including elastically deformable particles and mitotic cells, and 3) tracking dynamics of individual objects over time. Utilizing microdroplets, we demonstrated temporal tracking of an enzymatic reaction on a per-droplet basis in real time. An upstream fluidic mixing system is also used to create known concentrations and dilutions of fluorophores in droplets, which permits direct calibration of measured fluorescence intensity to fluorophores in solution.
Add to Calendar ▼2024-11-18 00:00:002024-11-20 00:00:00Europe/LondonInnovations in Flow Cytometry and Extracellular Vesicles 2024Innovations in Flow Cytometry and Extracellular Vesicles 2024 in Laguna Hills, CaliforniaLaguna Hills, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2024-11-19 15:30:002024-11-19 16:30:00Europe/LondonA Microfluidic Serial Cytometer to Estimate Per-Cell Uncertainty and Single Object Kinetic MeasurementsInnovations in Flow Cytometry and Extracellular Vesicles 2024 in Laguna Hills, CaliforniaLaguna Hills, CaliforniaSELECTBIOenquiries@selectbiosciences.com
