Miguel Conde's Biography

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Detection of Nanobodies® in Cell-based Homogeneous Assays

Thursday, 30 June 2011 at 09:00

Add to Calendar ▼2011-06-30 09:00:002011-06-30 10:00:00Europe/LondonDetection of Nanobodies® in Cell-based Homogeneous AssaysSELECTBIOenquiries@selectbiosciences.com

Nanobodies are isolated from immune blood samples through a library-based approach and tested for specificity in a variety of cellular and biochemical approaches. While biochemical setups are clean and fast, cell-based screens tend to be complex and time-consuming. The development of cell-based homogeneous assays reduced laborious experiments to simple mix and read setups. This, coupled with the development of potent image cytometry software, simplified the generation of physiologically relevant data. Here, we present results from two microplate cytometers used for fluorescence applications, FMAT™ and Mirrorball™, which allow the quantitative detection of Nanobody-binding in cell-based homogeneous assays.