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SELECTBIO Conferences Liquid Biopsies 2019

Yazhen Zhu's Biography

Yazhen Zhu, Assistant Project Scientist, David Geffen School of Medicine at UCLA

Dr. Yazhen Zhu is a Pathologist who obtained her MD and PhD training in the fields of Clinical Medicine and Pathology. She is currently an Assistant Researcher in the Department of Molecular & Medical Pharmacology at UCLA David Geffen School of Medicine, working on the development of non-invasive cancer diagnostic assays. Her interest remains in bridging the gap between translational medicine and unmet clinical needs in the field of molecular pathology and oncology.

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Quantitative Detection of Gene Rearrangements Using Circulating Tumor Cells Purified by a Covalent Chemistry-Enabled Platform

Thursday, 28 March 2019 at 13:00

Add to Calendar ▼2019-03-28 13:00:002019-03-28 14:00:00Europe/LondonQuantitative Detection of Gene Rearrangements Using Circulating Tumor Cells Purified by a Covalent Chemistry-Enabled PlatformLiquid Biopsies 2019 in Coronado Island, CaliforniaCoronado Island,

Recent research focus in the field of circulating tumor cells (CTCs) has moved beyond the simple capture or enumeration of CTCs and gravitated towards approaches that allow for in-depth molecular analysis. Unlike circulating tumor DNA or RNA which is highly fragmented and compounded by significant background, CTCs house intact genomic DNA and RNA,providing more reliable genetic information about tumors. Exploring the use of CTC-derived mRNA offers a non-invasive diagnostic solution for understanding underlying tumor biology, guiding treatment intervention, and monitoring disease progression. Recently, we demonstrated a new covalent chemistry-enabled CTC capture/release platform – “Click Chip”. This platform is designed by integrating bioorthogonal ligation-mediated CTC capture (sensitive and rapid) and disulfide cleavage-driven CTC release (mild, specific, and rapid) on nanostructured substrates to enable more efficient purification of pooled CTCs with high-quality intact CTC-derived mRNA. The CTC-derived mRNA was subjected to RT-droplet digital PCR (RT-ddPCR). ALK/ROS1 rearrangements were quantified using CTC-mRNA recovered by Click Chips and matched with those identified in biopsy specimen in late-stage ALK/ROS1 positive NSCLC patients. Moreover, copy numbers of ALK/ROS1 rearrangements in CTCs and CTC counts could be used together for evaluating ALK/ROS1-TKI treatment responses and disease progression. This streamlined diagnostic workflow is optimum for gene rearrangement detection and quantification based on high-quality intact CTC-derived mRNA.

Add to Calendar ▼2019-03-27 00:00:002019-03-29 00:00:00Europe/LondonLiquid Biopsies 2019Liquid Biopsies 2019 in Coronado Island, CaliforniaCoronado Island,