Daniel Chiu,
A. Bruce Montgomery Professor of Chemistry,
University of Washington
Daniel T. Chiu is currently the A. Bruce Montgomery Professor Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington. He is a member of the University of Washington’s Center for Nanotechnology, Neurobiology and Behavior Program, and the Cancer Consortium of the Fred Hutchinson Cancer Research Center. He has authored more than 180 publications and is the inventor on over 40 issued patents. Dr. Chiu obtained a B.A. in neurobiology and a B.S. in chemistry at the University of California, Berkeley in 1993, and a Ph.D. in chemistry from Stanford University in 1998.
High-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot Sorting
Monday, 13 December 2021 at 16:30
Add to Calendar ▼2021-12-13 16:30:002021-12-13 17:30:00Europe/LondonHigh-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot SortingLab-on-a-Chip and Microfluidics 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
This presentation describes the high-sensitivity isolation of rare cells, including circulating tumor cells and fetal cells, using fluorescence activated aliquot sorting. Here, a blood sample is divided into nanoliter volumes or aliquots, then analyzed and sorted based on the presence or absence of a rare cell. By performing two rapid, on the millisecond timescale, back-to-back sorting, we also demonstrate isolation of rare cells with high purity. Finally, we drastically increased the number of nucleated cells sorted by first concentrating peripheral blood mononuclear cells from whole blood, which translated into an equivalent blood volume analyzed by over an order of magnitude, from 8mL to over 100mL per experiment.
Add to Calendar ▼2021-12-13 00:00:002021-12-15 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics 2021Lab-on-a-Chip and Microfluidics 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-12-13 16:30:002021-12-13 17:30:00Europe/LondonHigh-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot SortingLab-on-a-Chip and Microfluidics 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
