Thomas Laurell's Biography

Thomas Laurell, Professor, Department of Biomedical Engineering, Lund University

Thomas Laurell received his PhD in electrical engineering in 1995 at Lund University and obtained a position as associate professor in 1998 at Lund University performing research on lab-on-a-chip technology interfaced to mass spectrometry proteomics and disease biomarker research as well acoustic manipulation of cells and particles in microfluidics systems. He holds a position as Professor in Medical and Chemical Microsensors since 2000 with a focus on Lab-On-A-Chip technologies in biomedicine at the Department of Biomedical Engineering, Div. Nanobiotechnology and Lab-on-a-chip (http://bme.lth.se/research-pages/nanobiotechnology-and-lab-on-a-chip/).

In 2005 Laurell co-founded the Chemical and Biological Microsystems Society, CBMS, the ruling body of the MicroTAS conference series and served as the President of CBMS, (www.cbmsociety.org) 2009-2017. He has also cofounded the Centre of Excellence in Biological and Medical Mass Spectrometry, a national infrastructure node at Lund University. In 2009 Laurell was appointed Distinguished Professor at Dongguk University, Dept. Biomedical Engineering, Seoul, Korea and he is an elected Fellow of School of Engineering, Tokyo University since 2015. Laurell is also an elected member of: The Royal Swedish Academy of Sciences, The Royal Academy of Engineering Sciences, and The Royal Physiographic Society. Laurell is a founder of AcouSort AB, http://www.acousort.com/

Acoustic Trapping Mediated High-Throughput (0.5 ml/min) Isolation of Extracellular Vesicles

Monday, 19 June 2023 at 12:15

Add to Calendar ▼2023-06-19 12:15:002023-06-19 13:15:00Europe/LondonAcoustic Trapping Mediated High-Throughput (0.5 ml/min) Isolation of Extracellular VesiclesLab-on-a-Chip and Microfluidics Europe 2023 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

Rapid and clinical scale isolation of extracellular vesicles (EV) from biofluids remains a major bottle neck in EV biomarker screening studies. Microfluidics has over the past years demonstrated several potential principles for EV isolation, however commonly suffering from limited capacity and/or sample throughput. In this perspective, acoustic nanoparticle trapping has emerged as an option for EV enrichment from biofluids where acoustic sound scattering, between microbeads and extra cellular vesicles, enrich EVs onto microbeads in a stationary cluster that is retained by the local acoustic field gradient. The technique has been used in investigations of EVs in blood plasma and urine. Traditionally this has been performed at a sample flow rate of ˜ 20 uL/min in a half wavelength acoustic standing wave trap, localized in a glass capillary, which when processing larger samples volumes, for increased biomarker sensitivity or when enriching EVs from dilute biofluids such as urine, suffer from extended processing times. To overcome this limitation we have developed an acoustic trapping unit, with ten standing wave nodes, that offers up to 40X increased trapping capacity and 25-40X increased sample processing flow rate. A typical sample flow rate of 500 uL/min enables rapid EV isolation and washing of milliliter volumes of urine as well as blood plasma in minutes prior to MS proteomic analysis. The proteome analysis of the acoustically enriched EV fraction displays clustering of proteins at elevated levels as compared to the input sample, suggesting a sub proteome specifically linked to the EVs. Furthermore, studies on EVs derived from pathogen activated platelets will be presented.

Add to Calendar ▼2023-06-19 00:00:002023-06-20 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2023Lab-on-a-Chip and Microfluidics Europe 2023 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

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