Rohit Karnik,
Associate Professor,
Massachusetts Institute Of Technology (MIT)
Rohit Karnik is Associate Professor of Mechanical Engineering at the Massachusetts Institute of Technology, where he leads the Microfluidics and Nanofluidics Research Group. His research focuses on the physics of micro- and nanofluidic flows and design of micro- and nanofluidic devices for applications in healthcare, energy systems, and bioseparation and analysis. He obtained his B. Tech. degree from the Indian Institute of Technology at Bombay in 2002, and his PhD from the University of California at Berkeley in 2006 under the guidance of Prof. Arun Majumdar. After postdoctoral work with Prof. Robert Langer at MIT, he joined the Department of Mechanical Engineering at MIT in 2007. Among other honors, he is a recipient of the Institute Silver Medal (IIT Bombay, 2002), NSF Career Award (2010), Keenan Award for Innovation in Undergraduate Education (2011), and DOE Early Career Award (2012).
Direct Separation and Analysis of Cells Mediated by Transient Molecular Interactions in Microfluidic Devices
Friday, 19 September 2014 at 14:00
Add to Calendar ▼2014-09-19 14:00:002014-09-19 15:00:00Europe/LondonDirect Separation and Analysis of Cells Mediated by Transient Molecular Interactions in Microfluidic DevicesLab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Multiple sample-processing steps present a challenge for the development of low-complexity devices for laboratory or point-of-care separation and analysis of cells. In this talk, I will discuss a new approach that can directly separate, enrich, or analyze cells with minimal or no sample processing requirements. We show that transient cell-surface adhesive molecular interactions can exert forces on the cells that can direct the trajectories of cells flowing through microfluidic devices. Such interactions occur in cell rolling, a physiological phenomenon involved in cell trafficking where transient molecular bonds are continuously formed and broken as the cell rolls on a surface under the action of hydrodynamic forces. Using this approach, we demonstrate separation of cells with high purity and efficiency in parallel microchannel devices, and direct separation of neutrophils from blood with ultrahigh enrichment in a neutrophil activation-dependent manner. We extend this approach to controllably contact mesenchymal stem cells with receptor-coated surfaces to quantify cell adhesion behavior by visualization of their trajectories in a “cell adhesion cytometer”, which can track changes in the cell phenotype. The results demonstrate the potential of the emerging technology of using transient cell-surface molecular interactions to directly separate and analyze cells for point-of-care diagnostics, isolation of rare cells, quality control of stem cells, and other applications.
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonLab-on-a-Chip, Microfluidics and Microarray World CongressLab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-09-19 14:00:002014-09-19 15:00:00Europe/LondonDirect Separation and Analysis of Cells Mediated by Transient Molecular Interactions in Microfluidic DevicesLab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
