Juan Santiago,
Charles Lee Powell Foundation Professor,
Stanford University
Juan G. Santiago received his MS and PhD in Mechanical Engineering from the University of Illinois at Urbana-Champaign in 1995. He holds the Charles Lee Powell Foundation professorship at Stanford and serves as Vice Chair of the Mechanical Engineering Department. His research includes the development of microsystems for on-chip biochemical analysis, microfluidic cell assay systems, two-phase flow devices, and capacitive deionization technologies. Applications of this work include molecular diagnostics, basic studies of cell biology, electronics cooling, and the production of drinking water. He is a Fellow of the American Physical Society, a Fellow of the American Society of Mechanical Engineering, and a Fellow of the American Institute for Medical and Biological Engineering. In 2022, he was elected to the American Academy of Arts and Sciences and to the National Academic of Inventors. He serves as the Editor-in-Chief of the journal Flow. His work is cited about 1500 times per year (Google Scholar h index of 86). He has authored and co-authored over 220 journal papers and 250 conference papers, and he is a named inventor in 58 issued patents (26 of which are currently licensed).
Life in the Shock Wave: Controlling DNA Reactions with Electric Fields
Tuesday, 29 September 2015 at 17:30
Add to Calendar ▼2015-09-29 17:30:002015-09-29 18:30:00Europe/LondonLife in the Shock Wave: Controlling DNA Reactions with Electric FieldsLab-on-a-Chip, Microfluidics and Microarrays World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
We use isotachophoresis (ITP) to create electric-field-driven shock waves of ion concentration inside microchannels. These waves are formed at the interface between a high mobility leading electrolyte (LE) and a low mobility trailing electrolyte (TE). Ionic species with mobilities bracketed by these electrolyte species focus at the LE-to-TE interface. For trace sample concentrations, multiple species mix and co-focus inside a single, order 10 µm wide wave front. Multiple reactants can be mixed and then pre-concentrated by more than 50,000x in a few minutes to accelerate chemical reactions. We apply this technique to extract and purify DNA or RNA targets from complex biological samples and to immediately co-focus these with synthetic DNA probes that we design. We pre-concentrate reactants by more than ~50,000x in a few minutes, and can complete in 30 sec chemical reactions which would normally take 4 days. Quantitation of the reaction product provides a sequence-specific detection scheme, and so the technique has applications to medical diagnostics and basic biological studies.
Add to Calendar ▼2015-09-28 00:00:002015-09-30 00:00:00Europe/LondonLab-on-a-Chip, Microfluidics and Microarrays World CongressLab-on-a-Chip, Microfluidics and Microarrays World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2015-09-29 17:30:002015-09-29 18:30:00Europe/LondonLife in the Shock Wave: Controlling DNA Reactions with Electric FieldsLab-on-a-Chip, Microfluidics and Microarrays World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
