Move Aside Culture! Rapid Molecular-based Quantification of Mycobacterium Tuberculosis in Sputum Takes Less Than a DayWednesday, 26 September 2012 at 11:15 Add to Calendar ▼2012-09-26 11:15:002012-09-26 12:15:00Europe/LondonMove Aside Culture! Rapid Molecular-based Quantification of Mycobacterium Tuberculosis in Sputum Takes Less Than a DayMolecular Diagnostics World Congress in San Diego, USASan Diego, USASELECTBIOenquiries@selectbiosciences.com Quantification of bacterial load in sputum samples from individuals with active tuberculosis is traditionally measured using solid agar. M. tuberculosis is very slow growing in culture, taking 3 weeks to produce visible colonies. RT-qPCR methods have not previously been used to quantify bacteria in sputum samples due to inhibitors and variability in RNA extraction. We describe an RNA-based assay which overcomes these problems. |