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SELECTBIO Conferences Molecular Diagnostics World Congress

Isobella Honeyborne's Biography



Isobella Honeyborne, Senior Research Associate, University College London

Isobella Honeyborne is a Senior Research Associate at University College London. Her current focus is on biomarkers of Mycobacterium tuberculosis. She earned her B.Sc (Hons) in Biology/Biochemistry at the University of Keele in 1999 and went on to develop interests in infectious disease. She spent time working on HIV-1 in South Africa and maintains strong links with groups in southern Africa. Her D.Phil, obtained in 2009, investigated CD8+ T cell responses to HIV-1 with particular interest in how particular HLA class I molecules are associated with failure or ability to contain the virus. A background in human immunology, in combination with study of prokaryotic biomarkers, allows a synergistic approach to developing molecular and other tools for Mycobacterium tuberculosis. The Molecular Bacterial Load (MBL) assay is an example of a bacteria-specific biomarker. She is also currently working on human miRNA and small RNA as both diagnostic and prognostic biomarkers of tuberculosis disease.

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Move Aside Culture!  Rapid Molecular-based Quantification of Mycobacterium Tuberculosis in Sputum Takes Less Than a Day

Wednesday, 26 September 2012 at 11:15

Add to Calendar ▼2012-09-26 11:15:002012-09-26 12:15:00Europe/LondonMove Aside Culture!  Rapid Molecular-based Quantification of Mycobacterium Tuberculosis in Sputum Takes Less Than a DayMolecular Diagnostics World Congress in San Diego, USASan Diego, USASELECTBIOenquiries@selectbiosciences.com

Quantification of bacterial load in sputum samples from individuals with active tuberculosis is traditionally measured using solid agar.  M. tuberculosis is very slow growing in culture, taking 3 weeks to produce visible colonies.  RT-qPCR methods have not previously been used to quantify bacteria in sputum samples due to inhibitors and variability in RNA extraction.  We describe an RNA-based assay which overcomes these problems.


Add to Calendar ▼2012-09-25 00:00:002012-09-26 00:00:00Europe/LondonMolecular Diagnostics World CongressMolecular Diagnostics World Congress in San Diego, USASan Diego, USASELECTBIOenquiries@selectbiosciences.com