Dr Fraikin developed Spectradyne’s initial Microfluidic Resistive Pulse Sensing technology as part of his PhD thesis work at UC Santa Barbara in the research group of Dr. Andrew Cleland. After graduating he received postdoctoral training in cancer cell biology and biochemistry and worked in industry developing microfluidics-based molecular diagnostics. Dr. Fraikin is a co-founder of Spectradyne and now serves as the company’s CEO.
Fluorescence Microfluidic Resistive Pulse Sensing: A Rapidly Emerging Method for Flow Cytometry at the Nanoscale
Thursday, 4 May 2023 at 12:00
Add to Calendar ▼2023-05-04 12:00:002023-05-04 13:00:00Europe/LondonFluorescence Microfluidic Resistive Pulse Sensing: A Rapidly Emerging Method for Flow Cytometry at the NanoscaleInnovations in Microfluidics 2023 in SeattleSeattleSELECTBIOenquiries@selectbiosciences.com
Nanoparticle-based biotechnologies including virus-mediated cell and
gene therapies, lipid nanoparticles (LNPs), and extracellular vesicles
(EVs) hold enormous promise as vaccines and therapeutics. These
revolutionary technologies derive their potency from payload-carrying
particles as small as 20 nanometers in diameter. At this size scale,
basic physical properties of the particles directly dictate the efficacy
and safety of the overall product and are therefore critical parameters
that must be measured to effectively develop and produce products based
on these materials. The realization of the potential of these medicines
is limited, however, by a lack of practical analytical tools for
measuring three key particle properties that critically impact dose and
bioavailability: Particle size, concentration, and payload. Despite new
developments that stretch the limits of its sensitivity, optical flow
cytometry is fundamentally limited in its ability to accurately detect
and measure nanoscale particles in heterogeneous samples. Fluorescence
Microfluidic Resistive Pulse Sensing (F-MRPS) is rapidly emerging as a
powerful alternative to optical flow cytometry that accurately measures
nanoparticle concentration, size, and payload in complex samples. F-MRPS
combines two orthogonal analytical techniques into a single
measurement: A cartridge-based electrical method for counting and
sizing particles (MRPS), and simultaneous high sensitivity
single-particle fluorescence measurements for quantifying payload. In
contrast with purely optical flow cytometry methods, the detection limit
of F-MRPS is not limited by light scattering intensity, and
measurements of particle size are completely independent of the
particle’s optical properties. F-MRPS is therefore uniquely suited to
analyzing complex heterogeneous samples and is rapidly being adopted by
industrial and academic users for quantification of EVs, virus and LNPs.
A technical overview of the F-MRPS technology will be presented,
together with measurement examples from key impact areas including virus
and gene therapy, extracellular vesicles, lipid nanoparticles (LNPs),
and liposomes. The fluorescence sensitivity of F-MRPS is demonstrated
with measurements of synthetic particles cross-calibrated to
NIST-traceable fluorescence intensity standards.
Add to Calendar ▼2023-05-04 00:00:002023-05-05 00:00:00Europe/LondonInnovations in Microfluidics 2023Innovations in Microfluidics 2023 in SeattleSeattleSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2023-05-04 12:00:002023-05-04 13:00:00Europe/LondonFluorescence Microfluidic Resistive Pulse Sensing: A Rapidly Emerging Method for Flow Cytometry at the NanoscaleInnovations in Microfluidics 2023 in SeattleSeattleSELECTBIOenquiries@selectbiosciences.com
