A Protein Array Platform with A New Approach to Labeling and DetectionFriday, 13 September 2013 at 10:45 Add to Calendar ▼2013-09-13 10:45:002013-09-13 11:45:00Europe/LondonA Protein Array Platform with A New Approach to Labeling and DetectionMicroarray World Congress in San Diego, CA, USASan Diego, CA, USASELECTBIOenquiries@selectbiosciences.com Porous nitrocellulose is well established as the substrate of choice for Reverse Phase Protein Arrays (tissue lysates), largely due to its high protein-binding capacity. Using a cytokine capture assay as a model, we demonstrate protein array performance in terms of dynamic range and limit of detection that meets or exceeds that reported for ELISA assays. For sensitive detection of proteins on porous nitrocellulose we developed complementary reagents and a fast and powerful microarray imager utilizing a low-noise, high-speed imaging camera. |