Akira Watanabe,
Assistant Professor,
Kyoto University
Akira Watanabe was born in Niigata Pref. Japan and graduated from Faculty of Engineering, Tokyo University of Science, and Graduate School of Engineering, the University of Tokyo. He received his Ph.D. in engineering from the University of Tokyo in 2003, and worked as a postdoctoral fellow at Research Center for Advanced Science and Technology, the University of Tokyo. He joined the faculty of Center for iPS Cell Research and Application (CiRA), Kyoto University in 2009, and is now an assistant professor of Prof. Shinya Yamanaka’s laboratory, and is also a head of Integrated Genomics and Epigenomics Core Facility of CiRA.
Genome and Epigenome Analysis of iPS Cells for Regenerative Medicine
Monday, 24 February 2014 at 16:00
Add to Calendar ▼2014-02-24 16:00:002014-02-24 17:00:00Europe/LondonGenome and Epigenome Analysis of iPS Cells for Regenerative MedicineNext Generation Sequencing: Research to Clinic in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Pluripotent stem cells are now suggested as an artificial source of tissues, and consequently it is necessary to be able to guarantee their safety in the human body after transplantation. However, both embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are produced after long-term culture, and thus harbor clone-to-clone variations in their DNA sequences and copy numbers as well as epigenetic profiles. It is therefore important to validate the quality of ES and iPS cells by genomic analyses. We aim to establish the standard method for evaluating iPS cells as a clinical-grade cell source by genome and epigenome analysis. We built the pipeline for both single-nucleotide variations (SNVs) and DNA copy number variations (CNVs), and performed exome and whole genome resequencing and compared between the original somatic cells and established iPS cells. We found not only clone-to-clone variations in iPS cell clones but also the heterogeneity in original somatic cells, indicating that comparison between original cells and established cells is essential for evaluating plulipotent stem cells. One of the iPS clones shows no non-synonymous mutations in exonic region. We also performed single cell transcriptome and genome analysis of iPS cells for evaluating quality of iPS cell clones that harbor heterogeneity . We introduce the strategy of genome and epigenome analysis for evaluating iPS cells for regenerative medicine using iPS cells.
Add to Calendar ▼2014-02-24 00:00:002014-02-25 00:00:00Europe/LondonNext Generation Sequencing: Research to ClinicNext Generation Sequencing: Research to Clinic in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-02-24 16:00:002014-02-24 17:00:00Europe/LondonGenome and Epigenome Analysis of iPS Cells for Regenerative MedicineNext Generation Sequencing: Research to Clinic in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
