Artis Galvanovskis,
Student,
Latvian Biomedical Research and Study Centre
Artis is an Msc. Student of molecular biology at the University of Latvia and a research assistant in Latvian Biomedical research and study center in Riga, Latvia. He currently works with establishing organ on chip systems made from alternative materials such as thermoplastics and other polymers that have reduced small molecule absorption and gas impermeability with the aim for drug and disease testing. Currently his focus is on human microbiota culture in an anerobic Gut on a chip model and Lung on a chip development from iPSCs. Artis also has experience with extracellular vesicle (EV) isolation from cell cultures and hollow fiber bioreactors.
Anaerobic Microbiota Cultivation by Gut on a Chip Developed from Highly Transparent Thermoplastic and Off-Stoichiometry Thiol-ene Polymer
Wednesday, 15 December 2021 at 17:00
Add to Calendar ▼2021-12-15 17:00:002021-12-15 18:00:00Europe/LondonAnaerobic Microbiota Cultivation by Gut on a Chip Developed from Highly Transparent Thermoplastic and Off-Stoichiometry Thiol-ene PolymerOrganoids and Organs-on-Chips 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
The gut microbiota and its products play a critical role in human health and in many physiological processes. An altered microbiota can lead to various diseases like autoimmune disorders, metabolic disorders and cancer. Therefore, there is an urgent need for in vitro test platforms for human microbiota, but the current research methods are limited. A promising, new method currently in microbiota research is the gut on-chip (GOC). However, the usual chips from polydimethylsiloxane are not suited for research of anaerobic microbiota due to their gas permeability and small molecular absorption. To tackle this problem, we developed GOC from highly transparent thermoplastic and Off-stoichiometry thiol-ene polymer, both materials have low gas permeability and low small molecule absorption. This approach allows us to create an oxygen gradient from an aerobic endothelial channel to the anaerobic channel representing the gut lumen. Therefore, this study aims to use alternative materials for GOC microfabrication and test this GOC for anaerobic microbiota cultivation. Additionally, we established anaerobic microbiota isolation from stool samples using GutAlive kit. Currently, we are optimizing the cultivation process of isolated microbiota from stool samples in anaerobic conditions in our GOC with CACO2 and HUVEC cells.
Add to Calendar ▼2021-12-13 00:00:002021-12-15 00:00:00Europe/LondonOrganoids and Organs-on-Chips 2021Organoids and Organs-on-Chips 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-12-15 17:00:002021-12-15 18:00:00Europe/LondonAnaerobic Microbiota Cultivation by Gut on a Chip Developed from Highly Transparent Thermoplastic and Off-Stoichiometry Thiol-ene PolymerOrganoids and Organs-on-Chips 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
