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SELECTBIO Conferences PCR & Next-Gen Sequencing

Carl Wittwer's Biography

Carl Wittwer, Professor, University of Utah

Carl Wittwer, MD, PhD is a professor of Pathology at the University of Utah. He introduced rapid cycle PCR in the early 1990s and was the primary inventor of the LightCycler system for real time PCR. In the 2000s, he also developed high resolution melting (HRM) for genotyping and variant scanning. He is currently chairman of BioFire Diagnostics (formerly Idaho Technology), still focused on increasing the speed and performance of targeted DNA diagnostics.

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Extreme PCR: Efficient Amplification in Less than One Minute

Tuesday, 29 April 2014 at 09:15

Add to Calendar ▼2014-04-29 09:15:002014-04-29 10:15:00Europe/LondonExtreme PCR: Efficient Amplification in Less than One

Extreme PCR combines higher primer and polymerase concentrations with cycle times of less than 2s, resulting in specific, high-yield amplification as demonstrated by gels, melting curves and real time analysis. Monovalent cations and dsDNA dyes inhibit, while DMSO enhances, polymerase extension.

Add to Calendar ▼2014-04-29 00:00:002014-04-30 00:00:00Europe/LondonPCR and Next-Gen