Mehran Mansouri's Biography

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A Barrier Tissue Model with an Ultrathin Nanoporous Membrane and Open-Well to Flow-Enhanced Reconfiguration Capability

Wednesday, 14 December 2022 at 18:30

Add to Calendar ▼2022-12-14 18:30:002022-12-14 19:30:00Europe/LondonA Barrier Tissue Model with an Ultrathin Nanoporous Membrane and Open-Well to Flow-Enhanced Reconfiguration CapabilityPoint-of-Care and Rapid Diagnostics 2022 in Long Beach, CaliforniaLong Beach, CaliforniaSELECTBIOenquiries@selectbiosciences.com

Despite the fact that they are unable to introduce physiological fluid flows, conventional membrane-based approaches (e.g., Transwell inserts) remain the gold standard to create barrier tissue models. Here, a barrier tissue platform with a 100 nm thick nanoporous membrane was developed that features reconfiguration capability between static and flow-enhanced culture modes. This platform allows users to conduct experiments using standard open-well protocols, and then add microfluidic capabilities to the culture when desired. As a demonstration, an endothelial barrier was established in a standard open-well format and then reconfigured to the microfluidic mode to induce shear alignment of cells. The results show that 89±4.9 % of cells aligned in the direction of flow while cells in the static condition remained randomly orientated. After shear-induced alignment of cells, the platform was reconfigured to the open-well format for mRNA isolation. The flow-stimulated cells up-regulated the expression of the shear-sensitive transcription factor KLF2 and the downstream production of eNOS 6.7x and 3.4x, respectively. To highlight high-resolution live imaging and flow capabilities, the device was reconfigured into microfluidic mode and different steps of leukocytes transmigration in response to chemical gradient were visualized. This platform enables more widespread use of flow-enhanced barrier models in bioscience laboratories.