Kennedy Okeyo,
Senior Lecturer, Institute for Frontier Life and Medical Sciences,
Kyoto University
Dr. Kennedy O. Okeyo is currently a senior lecturer at the Institute for Frontier Life and Medical Sciences, Kyoto University. He graduated with a PhD in mechanical engineering from Kyoto University, Japan, and worked briefly in a company thereafter. Prior to his current appointment, he worked as an assistant professor at the Department of Mechanical Engineering, The University of Tokyo, Japan. His current research interests includes tissue engineering, biomicrofluidics, and cellular biomechanics, with specific focus on stem cell function manipulation based on cell-material interactions.
Tuning Cell Orientation based on Localized Geometry Sensing during Cell Sheet Formation by the Micromesh Culture Technique
Tuesday, 12 November 2019 at 10:40
Add to Calendar ▼2019-11-12 10:40:002019-11-12 11:40:00Europe/LondonTuning Cell Orientation based on Localized Geometry Sensing during Cell Sheet Formation by the Micromesh Culture TechniqueCell and Gene Therapy Asia 2019 in Kobe, JapanKobe, JapanSELECTBIOenquiries@selectbiosciences.com
Cell sheets are self-assembled cellular constructs with potential application to regenerative medicine and cell-based therapy. Indeed, ongoing researches have demonstrated the potential application of cell-sheets in the regeneration of the cornea, esophagus, etc, with promising results. However, for application to oriented tissues like the heart and muscle tissues, cell sheets with clearly defined global cell orientation is required. However, presently available cell sheet technologies cannot meet this task. Moreover, since the most widely employed cell sheet fabrication technique involves the use of temperature-responsive culture vessels and other similar culture platforms, cell sheet damage during harvesting from such sticky culture platforms presents a major obstacle. In this talk, we present a simpler and easy-to-implement cell sheet fabrication approach which utilizes adhesion-limiting micromeshes as inducers of self-assembly mediated solely by cell-cell adhesion in a suspension. We demonstrate that tuning the mesh microstructure, such as mesh shape and size, can enable us to control whole-sheet cell orientation, a process which occurs naturally based on geometry sensing capability of cells. We also show that cell sheets can be generated in a suspension, eliminating the need for expensive harvesting strategies.
Add to Calendar ▼2019-11-11 00:00:002019-11-12 00:00:00Europe/LondonCell and Gene Therapy Asia 2019Cell and Gene Therapy Asia 2019 in Kobe, JapanKobe, JapanSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-11-12 10:40:002019-11-12 11:40:00Europe/LondonTuning Cell Orientation based on Localized Geometry Sensing during Cell Sheet Formation by the Micromesh Culture TechniqueCell and Gene Therapy Asia 2019 in Kobe, JapanKobe, JapanSELECTBIOenquiries@selectbiosciences.com
