Identifying a Predictive Signature of Neuronal Reactivity in Single Hippocampal NucleiWednesday, 3 October 2018 at 11:30  Add to Calendar ▼2018-10-02 00:00:002018-10-02 01:00:00Europe/LondonTitle to be Confirmed.Single Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.comActivity-induced remodeling of neuronal circuits is critical for memory formation. This process relies in part on transcription, but neither the rate of activity nor baseline transcription is equal across neuronal cell types. In this study, we isolated mouse hippocampal populations with different activity levels and used single nucleus RNA-seq to compare their transcriptional responses to activation. We found that 1 hr after novel environment exposure sparsely active dentate granule (DG) neurons had a much stronger transcriptional response compared to more highly active CA1 pyramidal cells and vasoactive intestinal polypeptide (VIP) interneurons. Activity continued to impact transcription in DG neurons up to 5 hr, with increased heterogeneity. By re-exposing the mice to the same environment, we identified a unique transcriptional signature that selects DG neurons for reactivation upon re-exposure to the same environment. These results link transcriptional heterogeneity to functional heterogeneity and identify a transcriptional correlate of memory encoding in individual DG neurons. |
Add to Calendar ▼2018-10-02 00:00:002018-10-03 00:00:00Europe/LondonSingle Cell Analysis Summit 2018Single Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
