Pulse Laser Activated Cell Sorter (PLACS) for High Speed Cell Sorting in MicrofluidicsFriday, 19 September 2014 at 19:15  Add to Calendar ▼2014-09-19 19:15:002014-09-19 20:15:00Europe/LondonPulse Laser Activated Cell Sorter (PLACS) for High Speed Cell Sorting in MicrofluidicsExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.comMicrofluidic-based fluorescence activated cell sorters (µFACS) have several advantages over conventional electrostatic-droplet-based cell sorters. A closed detection and sorting environment prevents aerosols that can potentially contaminate equipment, personnel, and subsequent sorting experiments. Although various on-chip µFACS mechanisms have been demonstrated, sorting live mammalian cells at high speeds with high sort purity and cell viability remains a challenge. Slow cell switching mechanisms and large fluid perturbation volumes are the two major factors limiting the throughput and purity of current microfluidic FACS devices. This talk presents a microfluidic based, high-speed and high-purity pulsed laser activated cell sorter termed PLACS which utilizes small volume liquid jets induced by pulse laser triggered cavitation bubbles for sample switching. A sorting throughput up to 23000 cells/sec with 90% sorting purity and 90% cell viability has been accomplished in a single microfluidic channel in a single stage. |
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonExosomes and Single Cell Analysis SummitExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
