Grace Zheng is the Applications lead for single cell products at 10x Genomics. We focus on developing new applications of high throughput single cell technology in basic and clinical research such as in the field of plant biology, immuno-oncology, cancer and cell atlas of different organisms. Before 10x Genomics, Grace studied the regulation of microRNAs and long noncoding RNAs at MIT and Stanford.
Quantitative Profiling of Large and Complex Single Cell Populations
Thursday, 5 October 2017 at 11:00
Add to Calendar ▼2017-10-05 11:00:002017-10-05 12:00:00Europe/LondonQuantitative Profiling of Large and Complex Single Cell PopulationsRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Understanding of complex biological systems requires the knowledge of their individual components. The ability to profile a large number of cells is becoming increasingly important for rare cell detection without complicated enrichment procedures, and for comprehensive classification of biological systems. However, existing single cell RNA-sequencing (scRNA-seq) methods have limited throughput, and computational analysis is not scalable.
The Chromium single cell system from 10x Genomics uniquely enables large-scale scRNA-seq studies. The updated microfluidics and biochemistry can capture >70% of loaded cells with a low doublet rate (<1% per 1000 cells), facilitating the profiling of precious and rare cell populations. 80k cells can be recovered in droplets per 7 min run, and the sequencing libraries can be made within 7 hours, enabling high sample and cell throughput. These libraries are compatible with the HiSeq4000 sequencer, currently the most cost effective sequencing platform provided by Illumina. The analysis pipeline, Cell Ranger, is optimized for efficient processing of sequencing data to produce per-cell gene expression matrices and an automated cell clustering analysis.
Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2017-10-05 11:00:002017-10-05 12:00:00Europe/LondonQuantitative Profiling of Large and Complex Single Cell PopulationsRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
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