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SELECTBIO Conferences Microfluidics & Organ-on-a-Chip Asia 2019

Ryuji Yokokawa's Biography



Ryuji Yokokawa, Associate Professor, Department of Micro Engineering, Kyoto University

Ryuji Yokokawa is an Associate Professor at Department of Micro Engineering, Kyoto University, Japan, and a Visiting Researcher at RIKEN Center for Biosystems Dynamics Research (BDR), Japan. He received his Ph.D. degree in Department of Electrical Engineering from The University of Tokyo in 2005. He was a visiting student in Prof. C.J. Kim's group, Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (2000–2001) and a visiting scholar with Prof. S. Takayama, Department of Biomedical Engineering, University of Michigan (2011–2012).

He has authored or co-authored more than 70 peer-reviewed journal and 120 conference papers, 1 book chapter, and has 5 patents issued or pending. He has served as a technical or organizing committee member in international conferences including Transducers, MicroTAS, IEEE NEMS, MEMS, Sensors and NANOMED.

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On-Chip Vasculature for Engineering Three-Dimensional Cell Culture Environment of Spheroids and Organoids

Friday, 15 November 2019 at 11:45

Add to Calendar ▼2019-11-15 11:45:002019-11-15 12:45:00Europe/LondonOn-Chip Vasculature for Engineering Three-Dimensional Cell Culture Environment of Spheroids and OrganoidsMicrofluidics and Organ-on-a-Chip Asia 2019 in Tokyo, JapanTokyo, JapanSELECTBIOenquiries@selectbiosciences.com

In vivo, healthy vasculature has a hollow structure to supply oxygen and nutrients to tissues. However, tissues cultured in vitro frequently lack such functional vasculature, and thus result in a necrotic core of the cell aggregate. To extend the culture period of the three-dimensional tissue, perfusable vasculature is crucial. It will contribute not only to the long-term culture for regenerative applications of tissues but also to deepen our understanding of organ morphogenesis.

In this presentation, I will explain a method to use angiogenic sprouts to connect the inside of a tissue model, spheroid or organoid, with microfluidic channels via vessel. Three types of tissue models were used: co-cultured spheroids of hLFs and HUVECs, and tri-cultured spheroids of hLFs, HUVECs, and tumor cells, and kidney organoids. This enables us to perfuse the inside of spheroids to deliver oxygen and nutrients. Moreover, some preliminary evaluation of tumor growth and drug evaluation will be presented.

We have extended the on-chip vascular formation method to a three-dimensional spheroid containing tumor cells and kidney organoids. Although we successfully optimized conditions to induce sprouting toward the co-cultured spheroid and found the lumen formation, it was not readily applicable to other cell types. Therefore, we took advantage of the angiogenic factors from hLFs even in the tumor spheroid model for vascularization. Future work includes many applications such as a high-throughput assay for drug screening and a long-term culture of organoids for studying organogenesis. The model provides a new assay platform for the tissue-culture with vasculatures at in vivo-like high cell density.


Add to Calendar ▼2019-11-14 00:00:002019-11-15 00:00:00Europe/LondonMicrofluidics and Organ-on-a-Chip Asia 2019Microfluidics and Organ-on-a-Chip Asia 2019 in Tokyo, JapanTokyo, JapanSELECTBIOenquiries@selectbiosciences.com