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SELECTBIO Conferences Extracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics

Haley Pugsley's Biography

Haley Pugsley, Manager and Senior Scientist, Luminex Corporation

Haley R. Pugsley, Ph.D., is a Manager, Senior Scientist for the Amnis Flow Cytometry products at Luminex Corporation-A DiaSorin Company. Haley received her Ph.D. in Analytical Chemistry from the University of Washington working under the direction of Professor Norman J. Dovichi. At the University of Washington, she used capillary electrophoresis to study protein fingerprinting of breast cancer tissue and created two reporter cell lines to investigate the expression of RecA in Deinococcus radiodurans. Dr. Pugsley did her postdoctoral fellowship at Hematologics, Inc. under the direction of Dr. Michael R. Loken where she focused on clinical flow cytometry and its use in diagnosing hematologic malignancies. Dr. Pugsley joined Amnis (now part of Luminex Corporation-A DiaSorin Company) in 2011 developing applications for the ImageStreamX Mk II, FlowSight and CellStream flow cytometers. During her time at Luminex, Dr. Pugsley has worked on many applications including autophagy, immunological synapse, algae biomass, cell signaling, an automated method for leukemia detection using multi-parametric analysis of imaging flow cytometry data and most recently extracellular vesicles.

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Analyzing Extracellular Vesicles With The Amnis® ImageStream®X Mk II High Gain Mode

Thursday, 25 February 2021 at 14:30

Add to Calendar ▼2021-02-25 14:30:002021-02-25 15:30:00Europe/LondonAnalyzing Extracellular Vesicles With The Amnis® ImageStream®X Mk II High Gain ModeExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics in Virtual ConferenceVirtual

The importance of EVs has become apparent, as they are key mediators of intercellular communication. However, quantifying and characterizing EVs in a reproducible and reliable manner is challenging due to their small size—exosomes range from 30 to 100 nm in diameter. Flow Cytometry is an attractive method for measuring EVs; however, this has been challenging since fl ow cytometers were designed to measure and detect cells. High Gain mode on the Amnis® ImageStream®X Mk II Imaging Flow Cytometer was designed to address the challenges of measuring particles that are much smaller than cells. This presentation demonstrates the improved small particle detection, including EVs, using this new High Gain mode.

Add to Calendar ▼2021-02-25 00:00:002021-02-26 00:00:00Europe/LondonExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and TherapeuticsExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics in Virtual ConferenceVirtual