Introduction to ISEV2015 Washington DCSunday, 26 October 2014 at 10:15  Add to Calendar ▼2014-10-26 10:30:002014-10-26 11:30:00Europe/LondonIsolation and Purification of EV and EV SubsetsISEV2014 Educational Event in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.comIsolation and Purification of EV and EV SubsetsSunday, 26 October 2014 at 10:30 Add to Calendar ▼2014-10-26 10:30:002014-10-26 11:30:00Europe/LondonIsolation and Purification of EV and EV SubsetsISEV2014 Educational Event in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.comIsolation and purification of extracellular vesicles and related particles, including viruses, has traditionally been performed by stepped centrifugation methods. Indeed, ultracentrifugation techniques remain standard in the field. However, the growing interest in EV as biomarkers and in therapeutics has been accompanied by a rapid proliferation of kit-based methods. Additionally, diverse combinations of approaches have been published in recent years. In this discussion, we will compare and contrast common methods for EV isolation and purification. The need to tailor the chosen approach to characteristics of the isolation medium and the ultimate research questions will be addressed. Finally, as an example, we will follow the crucial steps and variables in a representative workflow: from collection of a sample through EV purification and initial characterization. |
Add to Calendar ▼2014-10-26 00:00:002014-10-26 00:00:00Europe/LondonISEV2014 Educational EventISEV2014 Educational Event in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
