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SELECTBIO Conferences Advances in qPCR

's Biography



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Dr. Makrigiorgos is the Director of the Medical Physics and Biophysics Division of the combined Radiation Oncology Department at Brigham and Womens, Dana Farber and Children’s Hospitals, and Associate Professor at Harvard Medical School, Boston, USA. He is a Member of the Editorial Board of Clinical Chemistry and has published over 100 articles, reviews and book chapters. His research interests include the development of novel DNA technologies for molecular diagnostics with application in Oncology. He is the inventor of several novel PCR-based techniques for Molecular Diagnostics, including Balanced-PCR, Anti-primer-quenching real time PCR, Hairpin-PCR and COLD-PCR.

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COLD-PCR Enhances High Resolution Melting and Next Generation Technologies for Sensitive Detection of Low-Level Cancer Mutations

Thursday, 19 April 2012 at 16:30

Add to Calendar ▼2012-04-19 16:30:002012-04-19 17:30:00Europe/LondonCOLD-PCR Enhances High Resolution Melting and Next Generation Technologies for Sensitive Detection of Low-Level Cancer MutationsSELECTBIOenquiries@selectbiosciences.com

Utilizing the clinical and diagnostic potential of low-abundance mutations present within wild-type DNA is vital for several fields, including cancer biomarker development, prenatal diagnosis, and infectious diseases. We show that the combination of enhanced forms of real-time COLD-PCR with high resolution melting (HRM) as well as with next generation sequencing broadens the application of these powerful technologies for detecting low-level mutations in circulating DNA, circulating cells, FFPE, sputum or other bodily fluids, tumor margins and stromal cells.


Add to Calendar ▼2012-04-19 00:00:002012-04-20 00:00:00Europe/LondonAdvances in qPCRSELECTBIOenquiries@selectbiosciences.com