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SELECTBIO Conferences Sample Preparation and Analysis

Alexis Sauer-Budge's Biography

Alexis Sauer-Budge, Biotechnology Managing Scientist, Exponent, Inc., Adjunct Research Assistant Professor, Biomedical Engineering Dept, Boston University

Dr. Sauer-Budge received her B.S./M.S. in Chemistry from Stanford University and her Ph.D. in Biophysics from Harvard University. While at Harvard, she worked on developing methods for studying and controlling the passage of DNA through biological nanopores in the laboratory of Professor Daniel Branton. After completing her Ph.D., she joined a start-up company BioScale, Inc. developing infectious disease and protein diagnostics on bioMEMS resonating membrane platform technology. Dr. Sauer-Budge joined Fraunhofer CMI in 2007 to lead their biomedical group. Her research portfolio includes rapid diagnostics (point-of-care, microfluidics, biosensors, assay development), tissue engineering (bio-printing), medical devices (multi-functional surgical tools and wearable monitors), and bench-top instrumentation. Some of her recent projects include design and development integrated lab-on-a-chip molecular diagnostics for pathogen detection from physiological samples, isolation of pathogens from blood for integration with novel surface enhanced Raman spectroscopy that enables strain specificity, novel methods for rapid antibiotic susceptibility detection within minutes, novel surgical tools and coatings for implantable devices, wearable chemotherapy monitors, and handheld drug detection systems. Dr. Sauer-Budge also manages the beta-prototyping core for the NIH Center for Future for Technologies in Cancer Care - a translational research and engineering programs.

Dr. Sauer-Budge is currently Biotechnology Managing Scientist, Exponent, Inc.

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Isolation of Dilute Pathogens from Blood

Tuesday, 24 March 2015 at 18:00

Add to Calendar ▼2015-03-24 18:00:002015-03-24 19:00:00Europe/LondonIsolation of Dilute Pathogens from

Traditionally, bacterial pathogens in the blood have been identified using culture-based methods that can take several days to obtain results.  This can lead to physicians making treatment decisions based on an incomplete diagnosis contributing to patient morbidity.  To decrease diagnosis time, we are developing a novel sample preparation device for isolating and concentrating dilute bacteria from blood. Though commercial kits exist for the removal of blood from these samples, they typically capture only DNA, thereby necessitating the use of blood culture for antimicrobial testing. Here, we report a novel, scaled-up sample preparation protocol carried out in a new microbial concentration device. The process can efficiently lyse 10 mL of bacteremic blood while maintaining the microorganisms’ viability.  This talk will present the methodology and examples of integration with downstream detection technologies, including PCR, SERS, and standard culture.  Our sample preparation protocol holds great promise for the rapid diagnosis of bacteremia directly from a primary sample.

Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonSample Preparation and