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SELECTBIO Conferences RNA-Seq, Digital PCR, and Liquid Biopsies: Capturing Value from Circulating Biomarkers

Robert Kitchen's Biography

Robert Kitchen, Research Associate, Yale University

Robert R. Kitchen is a research associate in the laboratories of Mark Gerstein (dept. of Molecular Biophysics and Biochemistry) and Angus Nairn (dept. of Psychiatry) at Yale University, New Haven, Connecticut, USA. He received his undergraduate degree in astrophysics at the University of Edinburgh in 2007 where he remained to complete a PhD in computational biology & biostatistics in 2011. His research focus is in the field of bioinformatics, developing algorithms for the analysis of high-throughput functional genomic data, specifically relating to the evolution, development, and disorder of the mammalian brain.

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Computational Analysis of Exosomal Small-RNA-Seq Data

Tuesday, 22 March 2016 at 11:00

Add to Calendar ▼2016-03-22 11:00:002016-03-22 12:00:00Europe/LondonComputational Analysis of Exosomal Small-RNA-Seq

Overview of analysis of small RNAs by RNA-sequencing, including best practices for mitigating nuisance contaminants and special considerations for profiling extra-cellular RNAs (exRNAs). Based on these best practices we present the extracellular RNA processing toolkit (exceRpt), a software suite for the analysis of RNA-seq data obtained from both cellular and extracellular small-RNA preparations. We extend beyond existing analysis methods used to assess cytosolic micro-RNAs (miRNAs) to specifically address experimental issues that may arise in exRNA profiling, such as variable contamination of ribosomal RNAs, the presence of other smallRNA biotypes, and fragmented mRNA and long non-coding RNA transcripts.  We also discuss alignments to exogenous miRNAs in miRBase, as well as to exogenous ribosomal RNAs in RDP for comparison with studies of the microbiome.

Add to Calendar ▼2016-03-21 00:00:002016-03-22 00:00:00Europe/LondonRNA-Seq, Digital PCR, and Liquid Biopsies: Capturing Value from Circulating