Chaperone-Assisted Crystallography with DARPins

Thursday, 30 June 2011 at 14:45

Add to Calendar ▼2011-06-30 14:45:002011-06-30 15:45:00Europe/LondonChaperone-Assisted Crystallography with DARPinsSELECTBIOenquiries@selectbiosciences.com

A recurring effort to crystallize a particular protein is to bind it non-covalently to a specific helper binding protein – a crystallization chaperone. There is a wide range of tools available nowadays but their handling is often not as convenient as required. A valuable addition to the present repertoire of binding proteins is the designed ankyrin repeat protein (DARPin) technology. The potential of these novel binding proteins is illustrated by co-crystal structures with a variety of target proteins, including a membrane protein. The usefulness of DARPins in crystallization, the potential for phasing through DARPins and other recent approaches will be presented.