Alisa Morss,
Associate Professor,
Drexel University
Alisa Morss Clyne is currently an Associate Professor of Mechanical Engineering, with a courtesy appointment in the School of Biomedical Engineering, Science, and Health Systems, at Drexel University in Philadelphia, PA. Dr. Clyne is director of the Vascular Kinetics Laboratory, which investigates integrated mechanical and biochemical interactions among cells and proteins of the cardiovascular system. She is particularly interested in how endothelial cell mechano-transduction changes in a diseased environment, and how fluid shear stress and substrate mechanics affect biochemical binding kinetics, transport, and signaling. Dr. Clyne received her bachelor’s degree in Mechanical Engineering from Stanford University in 1996. She worked as an engineer in the GE Aircraft Engines Technical Leadership Program for four years, concurrently earning her Master’s degree in Mechanical Engineering from the University of Cincinnati. In 2006, she received her Doctorate in Medical and Mechanical Engineering from the Harvard-MIT Division of Health Sciences and Technology. In 2014, she completed the Executive Leadership in Academic Technology and Engineering (ELATE) program. Dr. Clyne received the NSF CAREER award in 2008, an AHA National Scientist Development Grant in 2010, and the BMES-CMBE Rising Star award in 2011. She has received research and educational funding from NSF, NIH, AHA, Department of Education, the Nanotechnology Institute, and the State of Pennsylvania, and she has published in diverse journals including Lab on a Chip, Journal of Biomechanics, Annals of Biomedical Engineering, Tissue Engineering, JRSI, PLOSOne, JBC, and Circulation. She is a member of ASEE, ASME, BMES, NAVBO, and SWE. Her teaching focuses on mechanical engineering applications in biological systems, and she founded several programs to enhance diversity within engineering.
Bioprinting Multicellular Structure to Advance Vascularized 3D Tissue Engineering
Tuesday, 27 March 2018 at 15:00
Add to Calendar ▼2018-03-27 15:00:002018-03-27 16:00:00Europe/LondonBioprinting Multicellular Structure to Advance Vascularized 3D Tissue EngineeringSELECTBIOenquiries@selectbiosciences.com
3D vascularized tissue engineering would enable mechanistic study of
healthy and diseased tissues, deliver a powerful platform for drug
screening, and potentially provide a replacement for diseased tissues.
The standard 3D tissue biofabrication process is layer-by-layer printing
of a bioink composed of cells within a matrix material. While this
process allows cells to be printed in a specific pattern to guide them
towards the desired 3D structure, it relies primarily on cellular
self-assembly into a 3D architecture that hopefully recapitulates the in
vivo tissue. Unfortunately, cellular self-assembly may take days or
weeks, may require complex spatial and temporal environmental cues, or
may not occur when two cell types are co-cultured together. Because of
these challenges, critical features of the tissue microenvironment
cannot be recapitulated, and therefore cell-cell interactions cannot be
studied in a physiologically relevant in vitro model. In this research,
we created a new bioprinting paradigm in which multicellular structures
that recapitulate in vivo architecture were used to create a 3D
vascularized breast cancer tissue model. The multicellular structures
were either grown in vitro prior to printing, or they were derived from
tissues (e.g., breast organoids). Our method decreases the time between
bioprinting and experimental assay from weeks to days; increases
physiological relevance by allowing the investigator to more precisely
control tissue architecture; and enables the use of primary human
tissues together with stromal cells and local extracellular matrix.
