Brian Eliceiri, PhD is a Professor in the Department of Surgery at the University of California San Diego. His research is focused on the role of extracellular vesicles (EVs) in regulating wound healing and immune cell crosstalk, with specific interests in the biogenesis of exosomes and how that relates to the cellular source of EVs, the cells that uptake EVs and the engineering of EV payloads to promote tissue repair. Using advanced approaches such as single vesicle flow for the analysis of wound fluid EVs, our lab has identified biologically important protein payloads that promote wound repair in animal models of impaired wound healing.
Mechanisms of Extracellular Vesicle Biogenesis that Regulate Wound Healing
Wednesday, 29 November 2023 at 11:15
Add to Calendar ▼2023-11-29 11:15:002023-11-29 12:15:00Europe/LondonMechanisms of Extracellular Vesicle Biogenesis that Regulate Wound HealingExtracellular Vesicles and Cell-Free RNAs 2023 in Laguna Hills, CaliforniaLaguna Hills, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Small extracellular vesicles (EVs) are important mediators of intercellular signaling that carry biologically active protein payloads relevant in wound healing. However, whether EVs are analyzed from wound fluid or other biological fluids, EVs are heterogenous, reflecting in part the release of EVs from different cell types. To address the central question of EV heterogeneity in parallel with the unmet need for in vivo sources of EVs with low platelet contamination, we have developed a macroporous scaffold for the subcutaneous implantation and subsequent collection of EVs that is applicable for the study of EV activity in any defined mouse model. Using polyvinylalcohol (PVA) sponges as the scaffold, we first show that the rapid infiltration of immune cells of hematopoietic origin is accompanied a substantial and heterogenous population of EVs. Second, we show how single vesicle flow cytometry (vFC) addresses the heterogeneity challenge by quantifying the expression of surface markers that map to specific subpopulations of EVs relevant to cellular origin. Third, we show how in vitro two-dimensional cell culture, although common, introduces significant bias in EV release that is addressed with the PVA scaffold in vivo model. Together these studies, define a novel model establishing the biochemical basis and biological activity of EVs in the biology of wound healing.
