SELECTBIO - Microfluidics & Organ-on-a-Chip Asia 2019 Biographies

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High Throughput Error-Correction Code DNA Sequencing

Thursday, 14 November 2019 at 15:30

Add to Calendar ▼2019-11-14 15:30:002019-11-14 16:30:00Europe/LondonHigh Throughput Error-Correction Code DNA SequencingMicrofluidics and Organ-on-a-Chip Asia 2019 in Tokyo, JapanTokyo, JapanSELECTBIOenquiries@selectbiosciences.com

Eliminating errors in next-generation DNA sequencing has proved challenging. I am going to talk about our newly developed error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory–based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage. We showed that, when combined with a fluorogenic SBS chemistry with raw accuracy of 98.1%, ECC sequencing provides single-end, error-free sequences up to 200 bp. ECC approaches should enable accurate identification of extremely rare genomic variations in various applications in biology and medicine.

Yanyi Huang, Professor, Peking University

Professor Yanyi Huang is Professor of Analytical Chemistry at Peking University, and holds Principle Investigator positions in Biomedical Pioneering Innovation Center (BIOPIC), Peking-Tsinghua Center for Life Sciences at Peking University, and Institute for Cell Analysis at Shenzhen Bay Laboratory. He received his BS and ScD degrees from Peking University, and conducted his postdoc research at Caltech and Stanford University. He is Fellow of Royal Society of Chemistry. He is working on technology development for integrative biology researches, especially the new methods for genomic sequencing and large scale microfluidics.