Improving sigmoidal fitting of real-time PCR curves

Wednesday, 5 September 2012 at 14:45

Add to Calendar ▼2012-09-05 14:45:002012-09-05 15:45:00Europe/LondonImproving sigmoidal fitting of real-time PCR curves SELECTBIOenquiries@selectbiosciences.com

A major drawback of fitting sigmoidal models to real-time PCR data is the low performance in the noisy baseline region, making the extrapolation of F0 (fluorescence at cycle 0) inaccurate. We will show that modifications such as variance-weighted fitting, robust nonlinear fitting and the application of linear-quadratic-sigmoidal hybrid models can substantially increase the accuracy of deduced parameters such as F0, efficiency and threshold cycle.