|
Held in conjunction with Lab-on-a-Chip European Congress
27 Mar 2012, at 09:00-12:30 in Edinburgh, Scotland
Microarray technology is a mature technology that has gone from being a research instruments to being used in diagnostics. In the course, basic concepts of microarray technology will be described regarding genotyping, gene expression and protein arrays. Application of these technologies within e.g. cancer classification, viral and bacterial diagnostics, biomarker discovery, and stem cell biology will also be described. Using array technology in diagnostics to save time, reagents and to improve the diagnostic power puts however novel demands on the quality of microarray technology. This demand is having a large impact on all steps to create and use microarrays. The course will highlight strengths and weaknesses of current microarray technology and principles in relation to diagnostics but also high throughput sequencing methods. The latter technology is challenging DNA microarrays as the method of choice for genetic and gene expression analysis.
Topics and Course Organisation
- Microarray principles and technology
- Probe design
- Fabrication
- Sample preparation
- Hybridization
- Detection
- Data analysis
- Genotyping
- Viral/bacterial diagnostics
- SNP detection
- Allele specific hybridization
- Primer extension reactions
- Comparative genomic hybridization and application in cancer genetics
- Comparison to high throughput sequencing
- Application in point of care devices
- Gene expression analysis
- Principles of gene expression analysis
- Importance of sample preparation and sample purity on results
- Applications
- Cancer diagnostics
- Biocompatibility
- Principles of data analysis
- Validation and quality control
- Comparison to high throughput sequencing “RNA Seq”
- Protein microarrays
- Classifications and definitions
- Gabrication issues
- Applications
- Antibody arrays for cancer subtyping and biomarker discovery
- Proteome arrays for analysis of protein-protein interactions and protein-small molecule interaction
- Protein microarray for screening stem cells responses
- Examples of protein microarrays in point of care devices inclusing lateral flow devices
|
 Martin Dufva, Associate Professor, Technical University of Denmark
|
|
