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Held in conjunction with NGS, SCA, Mass Spec: The Road to Diagnostics
28 Sep 2015, at 18:30-21:00 in San Diego, California, USA
Introduction
The goal of this short course is to introduce participants into the burgeoning area of single cell analysis (SCA) using lab-on-a-chip (LOAC) as the operational platform to secure biological and/or biomedical information from various cell types. This short course will be broken down into two general areas: (1) Basic fabrication protocols and materials selection for LOAC applications; and (2) Application of LOAC technologies for single cell analyses. The topic areas in each section are outlined below. Topics Covered
Basic Fabrication Protocols and Materials Selection - Introduction to LOAC technologies; what can they provide with respect to single cell analyses and comparison to benchtop procedures.
- Differences between microfluidics and nanofluidics for LOAC
- Materials for LOAC
Fabrication Technologies- Micromilling
- Lithography
- Dry/wet etching
- Molding
- Fabrication of structures below the diffraction limit (<100 nm)
- Assembly of devices for fluidic applications
Metrology and Fluid Characteristics in Microfluidics and Nanofluidics
- Characterizing structures above and below the diffraction limit
- Electrokinetic versus hydrodynamic flow
- Basic fluid and particle transport
Application of LOAC technologies for Single Cell Analysis (SCA)
- Basic characteristics of biological cells – what does single cell analyses buy you?
- Challenges with single cell analysis – molecular content of single cells
- Analyzing single cells – basic processing steps
- Single cell lysis techniques
- Imaging single cells using LOAC technologies
- Separating single cells (dielectrophoresis, affinity selection)
- Analyzing single cells in two-phase flows (droplet microfluidics)
- Digital PCR
- Examples of single cell analysis using microfluidics
- Circulating tumor cells (CTCs)
- Pre-natal diagnostics using circulating fetal cells
- Single cell sequencing using Next Generation Sequencing (NGS)
Delegates receive all presentations and course materials as electronic files allowing utilization in R&D presentations, business plans and investor presentations. This dinner training course includes dinner for delegates.
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 Steve Soper, Foundation Distinguished Professor; Director, Center of BioModular Multi-scale System for Precision Medicine, Adjunct Professor, Ulsan National Institute of Science & Technology, The University of Kansas
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