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Held in conjunction with Lab-on-a-Chip & Microfluidics
16 Mar 2015, at 13:00-17:00 in Berlin, Germany
Who should attend?
The course is suitable for scientists, technicians and engineers who would like to learn (more) about microfluidics and its applications in the area of life sciences. Researchers who are interested in working with microfluidic devices will acquire a comprehensive overview of the field. The course will also provide an opportunity for researchers who already have experience with microfluidic devices but who would like to update or broaden their knowledge on recent developments in bio-applications.
Learning Objectives 1. Understand the benefits and limitations of miniaturisation and learn how microfluidic devices can be fabricated, assembled and operated. 2. Understand the behaviour of fluids in microchannels and learn how this has led to methods of pumping, mixing and detection that can be quite different from those used on the larger scale. 3. Gain a broad overview over the applications of microfluidic devices in the area of life sciences including DNA and protein analysis as well as recent developments in cell and tissue analysis. 4. Understand the challenges and recent developments towards integrated and portable analysis systems (microTAS) that can be used at the point-of-care for medical applications, at-the-scene for forensic applications or in-the-field for environmental and biodefence applications.
Topics and Course Organisation
Principles of Microfluidics and Construction of microTAS
1. Benefits of miniaturising fluid handling for (bio)chemical reactions for the separation of sample mixtures integration and automation, micro total analysis systems (microTAS)
2. Flow behaviour in microchannels laminar and turbulent flow diffusion multi-phase flow
3. Handling of fluids components for fluid handling pumping (hydrodynamic, electroosmotic) mixing (passive and active) valves and other means of flow stream control
4. Detection in microfluidic devices optical (fluorescence, chemiluminescence) electro-chemical spectroscopic and spectrometric
5. Fabrication of Microfluidic Devices materials and biocompatibility silicon and glass devices polymer devices interfacing and packaging
Applications of Microfluidic Devices in Bioanalysis
1. DNA analysis DNA separation on-chip polymerase chain reaction DNA hybridisation assays
2. Protein analysis on-chip immunoassays pre-concentration of proteins protein separation microchips coupled to mass spectrometers
3. Cell and tissue analysis handling of cells in microfluidic devices single cell analysis parallel analysis on cell arrays on-chip tissue analysis
4. Integrated devices for point-of-care or in-the-field analysis microfluidic devices for medical point-of-care applications microfluidic devices for forensic applications microfluidic devices for biodefence applications
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