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3 for 2 Offer SELECTBIO Conferences Microfluidics and LAB-ON-A-CHIPFlow Chemistry India 2018Flow Chemistry Europe 2018BioEngineering 2018Circulating Biomarkers World Congress 2018ePoster Award Prize

Principles and Applications of Microfluidics in the Life Sciences



Held in conjunction with Lab-on-a-Chip World Congress

24 Sep 2012, at 09:00 - 12:30 in San Diego, USA

Price:



Who should attend?

The course is suitable for scientists, technicians and engineers who would like to learn (more) about microfluidics and its applications in the area of life sciences. Researchers who are interested in working with microfluidic devices will acquire a comprehensive overview of the field. The course will also provide an opportunity for researchers who already have experience with microfluidic devices but who would like to update or broaden their knowledge on recent developments in bio-applications.

Learning Objectives

  1. Understand the benefits and limitations of miniaturisation and learn how microfluidic devices can be fabricated, assembled and operated.
  2. Understand the behaviour of fluids in microchannels and learn how this has led to methods of pumping, mixing and detection that can be quite different from those used on the larger scale.
  3. Gain a broad overview over the applications of microfluidic devices in the area of life sciences including DNA and protein analysis as well as recent developments in cell and tissue analysis.
  4. Understand the challenges and recent developments towards integrated and portable analysis systems (microTAS) that can be used at the point-of-care for medical applications, at-the-scene for forensic applications or in-the-field for environmental and biodefence applications.

Topics and Course Organisation

Principles of Microfluidics and Construction of microTAS

  1. Benefits of miniaturising fluid handling
    for (bio)chemical reactions
    for the separation of sample mixtures
    integration and automation, micro total analysis systems (microTAS)
    microfluidics versus nanofluidics
  2. Flow behaviour in microchannels
    laminar and turbulent flow
    diffusion
    multi-phase flow
  3. Handling of fluids
    components for fluid handling
    pumping (hydrodynamic, electroosmotic)
    mixing (passive and active)
    valves and other means of flow stream control
  4. Detection in microfluidic devices
    optical (fluorescence, chemiluminescence)
    electro-chemical
    spectroscopic and spectrometric
    thermal lens microscopy
  5. Fabrication of Microfluidic Devices
    materials and biocompatibility
    silicon and glass devices
    polymer devices
    interfacing and packaging

Applications of Microfluidic Devices in Bioanalysis

  1. DNA analysis
    DNA separation
    on-chip polymerase chain reaction
    DNA hybridisation assays
  2. Protein analysis
    on-chip immunoassays
    pre-concentration of proteins
    protein separation
    microchips coupled to mass spectrometers
  3. Cell and tissue analysis
    handling of cells in microfluidic devices
    single cell analysis
    parallel analysis on cell arrays
    on-chip tissue analysis
  4. Integrated devices for point-of-care or in-the-field analysis
    microfluidic devices for medical point-of-care applications
    microfluidic devices for forensic applications
    microfluidic devices for biodefence applications

Nicole Pamme

Nicole Pamme, Professor, The University of Hull