Diagnosis of Brain TB: How Small Steps Leads to Big Changes
Rajpal Singh Kashyap,
Senior Scientist (Medical) and Head,
Central India Institute of Medical Sciences
Over the past few decades, analyses of biomarkers have attracted attention with respect to a variety of infectious and non infectious disorders. With the increased interest in clinical proteomics, comparative investigations with respect to differential protein expression, advanced molecular technology has become more common than ever before for the diagnostic and prognostic assessment of disease states. In this presentation, I am going to talk about, how one can identify the markers specific to brain TB (Tuberculous meningitis) using proteomic techniques one and two dimensional electrophoresis and immunological and molecular tools and also use of these biomarkers to develop diagnostic kit. In our laboratory we have used proteomic, immunological and molecular tools and identified several new biomarkers which will be useful for the development of sensitive and specific tests for the prediction and/or early diagnosis of Brain TB and also to understand the pathogenesis of disease. One of the least understood issues in the pathogenesis and pathophysiology of Brain TB is how microorganisms cross the blood-brain barrier (BBB), which separates brain interstitial space from blood and is formed by the tight junctions of brain microvascular endothelial cells (BMEC).
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