Next-Gen Sequencing Agenda

Registration

Coffee and Networking in Exhibiton Hall

High Sensitivity Detection of Colorectal Cancer Mutations using Third Generation Single Molecule Sequencing
Giancarlo Russo, Bioinformatics and Biostatistics; Next Generation Sequencing Expert, ETH Zurich / University of Zurich, United Kingdom

The advent of the PacBio RS, whose random error model and circular consensus algorithm allow for base calls quality which are orders of magnitude better than current NGS platform, opens up new frontiers in the detection of very low frequency mutations.  This talk will focus on a recent application to colorectal cancer.

Reference Human Epigenomes
Martin Hirst, Head, BC Cancer Agency, Canada

As part of the NIH Reference Epigenome Project (http://www.roadmapepigenomics.org) and Canadian Epigenetics and Environment Health Research Consortium (http://www.cihr-irsc.gc.ca/e/43734.html) our group is engaged in the generation of comprehensive reference epigenomes from primary human tissues and cells.

Technology Spotlight:
Automating the Tricky Part of NGS – Size Selection and More
Stefan Overkamp, Product Specialist/Product Manager, Beckman Coulter

Lunch and Networking in Exhibition Hall

Poster Viewing Session

Deep Sequencing, microRNA/Other Novel Small Non-coding RNA, and Renal Cell Carcinoma Metastasis
Huiqing Wu, Assistant Professor, City of Hope National Medical Center and Beckman Research Institute, United States of America

Whole genome small RNA deep sequencing studies using formalin-fixed paraffin-embedded clinical renal cell carcinoma samples. Discovery and characterization of novel small non-coding small RNAs derived from tRNAs, rRNAs, snoRNAs and other RNAs/RNA fragments by deep sequencing studies of Ago2-coprecipitated small RNAs and clinical renal cell carcinoma tissue cohort.

The Genetics of Orphan Diseases
Gijs van Haaften, Group Leader, University Medical Center Utrecht, Netherlands

In this presentation I will present different approaches that I apply in my lab to identify the genetic basis of several orphan diseases, for example we recently identified autosomal dominant mutations in a K-ATP channel to cause Cantu syndrome.

Coffee and Networking in Exhibiton Hall

The Epigenetics of Pluripotency in Mouse Ground State Embryonic Stem Cells (ESCs)
Hendrik Marks, Post Doctoral Researcher, Nijmegen Centre for Molecular Life Sciences/Radboud University, Netherlands

ESCs have the potential to differentiate into all somatic cell lineages, referred to as pluripotency, and can propagate indefinitely. To understand the mechanisms acting in these unique cells, I use an integrative approach of NGS applications (ChIP-Seq, WGBS, (nc)RNA-Seq, DNAseI-Seq), which will be presented at this meeting.

Automating Microbial Annotation and Analysis
Lisa Crossman, Project Leader, University of East Anglia, United Kingdom

Microbial genome processing pipelines range from the fastest and most minimal to the longer and more in-depth.  Processing steps at TGAC, Norwich, UK follow different options for particular project types dependent on biological variation.

MutAid- A Comprehensive Software Solution for Efficient Molecular Genetic Analyses of NGS Data
Andreas Weinhaeusel, Senior Scientist, Austrian Institute of Technology GmbH, Austria

End of Day One

Translating Epigenetic Alterations in Leukemia into Biomarkers of the Disease and for Target Therapies with DNA Demethylating Drugs
Michael Schnekenburger, Team Leader, Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Luxembourg

Aberrant DNA methylation patterns are a common hallmark of tumor cells. We will discuss the translational potential of these epimutations as cancer biomarkers and possible therapeutic strategies related to DNA demethylation.

Coffee and Networking in Exhibiton Hall

Genes for Sequencing: Methods and Applications in Cancer Genomics
James Hadfield, Head, Genomics Core Facility, Cancer Research UK, United Kingdom

Many methods are available to capture specific genes for next-generation sequencing. This presentation will present an overview and comparison of these methods, discuss their pro's and con's and present data from cancer genomics studies using NGS gene capture.

Automation Challenges in Library Construction for Next Generation DNA Sequencing
Robin Coope, Group Leader and CoDirector, British Columbia Cancer Agency Genome Sciences Centre, Canada

The BC Cancer Agency's Genome Sciences Centre has built high throughput sequencing pipelines for challenging molecules, particularly miRNA and mRNA, and now, rapid turnaround clinical sequencing.  To achieve this we have developed several novel tools including a 96-channel robot for gel based size selection.  This platform will be discussed along with highlights of other novel approaches to sequencing pipeline bottlenecks.

Lunch and Networking in Exhibition Hall

Poster Viewing Session

High-throughput Automated ChIPseq
Sarah Aldridge, Senior Staff Scientist, Cambridge Research Institute, United Kingdom

We have set-up a high-throughput functional genomics annotation pipeline centred around automated chromatin immunoprecipitation (ChIP).  I will describe this automated method and discuss how our lab uses it as part of a systems level, integrative approach to explore the control and evolution of gene expression.

Next-Generation-Sequencing Technologies in Genetic Diagnostics
Jiri Ködding, Head of Next-Generation Sequencing, CeGaT, United Kingdom

CeGAT will present their NGS approach to Diagnostic sequencing and will include 1-3 slides on Covaris LE220 multiparallel library preparation as part of their HT-process.

Coffee and Networking in Exhibiton Hall

Complete Automation of NGS Workflows
Russell Green, Product Manager EMEAI, Beckman Coulter UK Ltd, United Kingdom

An overview of the broad spectrum of NGS sample preparation methods that have been developed and implemented on the Biomek liquid handlers.  This includes everything from nucleic acid extraction and library construction using SPRI chemistries to library construction and target sequence capture.

Close of Conference