Single Cell Analysis Europe 2014 Agenda

Registration

Tracking Stem Cell Fate Choices at Single Cell Level
Adrian Ranga, Senior Scientist, Ecole Polytechnique Federale de Lausanne, Switzerland

We tackle the problem of complexity in the stem cell niche by developing novel platforms and tools which enable us to perform high-throughput combinatorial methods to assess cell fate at single cell resolution.

Coffee & Networking in Exhibition Hall

Probing Cellular Heterogeneity in Cytokine-secreting Cells using High-throughput Droplet-based Microfluidics
Jurjen Tel, Postdoctoral Fellow, Radboud University Nijmegen Medical Centre, Netherlands

In this presentation I will highlight the necessity of single cell analysis tools and I will discuss data that were obtained by our developed microfluidic platform to detect cytokine secretion by single cells.

Towards Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health
David Klug, Co-Founder/Professor, Imperial College London, United Kingdom

The latest methods in use in my lab for single cell analysis are presented including preliminary results from clinical samples.

Lunch & Networking in Exhibition Hall

Poster Viewing Session

Free Workshop
Takara Clontech Lunchtime Workshop - Improved cDNA Library Generation for Transcriptome Analysis from a Single Cell
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Parallel Single Cancer Cell Analysis
Séverine Le Gac, Professor, Applied Microfluidics for Bioengineering Research, MESA+ Institute for Nanotechnology, University of Twente, Netherlands

Here, we present the development of microfluidic tools to achieve essential steps of a parallel single cell analysis protocol, namely for the large-scale trapping of individual cells in dedicated structures, for in situ cell staining for, e.g., cancer cell identification in mixed cell populations, electrical lysis of individual cells followed by the retrieval of their content, and mapping of point mutations after on-chip whole genome amplification and off-chip sequencing.

Single Cell Analysis of Rare Cells
Thomas Kroneis, Head, Medical University of Graz, Austria

The analysis of rare cells longs to focus on two major issues, namely the enrichment for and the detection and confirmation of candidate rare cells allowing for reasonable downstream analysis. In my talk I will address both issues and point out possible pitfalls in the context of rare cell analysis based on our experience in the analysis of circulating fetal cells, maternal microchimerism and circulating tumor cells.

Coffee & Networking in Exhibition Hall

Detection and Expression Profiling of Tumor Initiating Cells (TIC) Among the Peripherally Circulating Epithelial Tumor Cells from Patients with Breast Cancer
Katharina Pachmann, Professor, University of Jena, Germany

Among the peripherally circulating tumor cells a variable fraction is able to express stem cell and adhesion properties and can be grown into tumor spheres, a property ascribed to cells capable of initiating tumors and metastases.

Taking Single Cell Expression Profiling to New Dimensions
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

In the first part of my talk I will describe high throughput single cell profiling of astrocytes from mouse brains performed over time after induced trauma. The profiling reveals how the brain responds to the injury, activating the astrocytes to attempt repair. Expression of many genes, measured in large number of cells, over time is a so called multiway study, from which most valuable information about the molecular processes involved and expression pathways active can be extracted with powerful multivariate tools such as dynamic PCA available in the GenEx software. In the second part of my talk I will present single cell multianalyte profiling, showing how DNA, mRNA, microRNA, lncRNA, and protein can be measured in the same cell.

Roundtable Discussion
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

• What contributes to single cell heterogeneity?

• (Why) are mRNA and protein expression bursts at different time scales?

• How do we define cell type? Can single cell profiling help?

• Single cell analysis in clinical practice? What´s needed?

Natural Killer Cell Immune Surveillance Studied at the Single Cell Level
Bjorn Onfelt, Associate Professor, Karolinska Institute, Sweden

Each individual has a heterogeneous pool of natural killer (NK) cells consisting of cells with varying ability to kill virus-infected or tumor cells. Here, this heterogeneity is dissected by studying NK cell migration dynamics and cytotoxic response at the single cell level.

Coffee & Networking in Exhibition Hall

Single Cell Analysis of Cellular Communication and Signaling Pathway Activity
Ola Soderberg, Associate Professor/Senior Lecturer, Uppsala University, Sweden

In the presentation I will describe methods developed in our lab, in situ PLA and padlock probes, and describe how these can be used to monitor signaling networks activity in fixed tissue sections.

Visualizing Structural Variations Of Single DNA Molecules In A Nanofluidic Device
Rodolphe Marie, Assistant Professor, Technical University of Denmark, Denmark

A nanofluidic device is used to image Mb-long DNA molecules from human at full stretching. We demonstrate how fluorescence barcodes are used to map single molecules to the hg18 reference and study structural variations of single copies of the genome.

Lunch & Networking in Exhibition Hall

Poster Viewing Session

Biological Single Cell Analysis
Andreas Schmid, Professor, Technische Universitat Dortmund, Germany

The biological analysis of single cells reveals spatial and temporal dynamics of the minimal unit of biological function- the cell. The presentation will focus on advanced lab-on-a-chip solutions for the study of living and isolated single cells.

Rapid Development of Small-Molecule Producing Bacteria using Metabolite Sensors and Recombineering
Stephan Binder, Post Doctor, Forschungszentrum Jülich Gmbh, Germany

We developed individual metabolite sensors to monitor cytosolic concentrations of L-lysine, L-histidine, L-arginine and further amino acids in C. glutamicum and E. coli. This methodology enables a number of novel FACS-based applications, including high-throughput isolation of individual producers from large mutant populations or in-vivo site-directed saturation mutagenesis using recombineering.

Close of Conference, Coffee & Networking in Exhibition Hall