John Wikswo,
Gordon A. Cain University Professor, A.B. Learned Professor of Living State Physics; Founding Director, Vanderbilt Institute for Integrative Biosystems,
Vanderbilt University
John Wikswo is the Gordon A. Cain University Professor at Vanderbilt University and is the founding Director of the Vanderbilt Institute for Integrative Biosystems Research and Education. Trained as a physicist, he received his B.A. degree from the University of Virginia, and his PhD. from Stanford University. He has been on the Vanderbilt faculty since 1977. His research has included superconducting magnetometry, the measurement and modeling of cardiac, neural and gastric electric and magnetic fields, and non-destructive testing of aging aircraft. His group’s current work on organ-on-chips focuses on the development of intelligent well plates that serve as perfusion controllers, microclinical analyzers, and microformulators; developing a blood-brain-barrier and a cardiac tissue construct on a chip; and integrating multiple organs to create a milli-homunculus from coupled organs on chips. As a tenured member of the Departments of Biomedical Engineering, Molecular Physiology & Biophysics, and Physics & Astronomy, he is guiding the development of microfabricated devices, optical instruments, and software for studying how living cells interact with each other and their environment and respond to drugs, chemical/biological agents, and other toxins, thereby providing insights into systems biology, physiology, medicine, and toxicology. He has over 250 publications, is a fellow of seven professional societies, and has received 39 patents.
Pumps, Valves, and Fluidic Connectors for Automating and Integrating Microphysiological Systems
Monday, 14 October 2019 at 09:00
Add to Calendar ▼2019-10-14 09:00:002019-10-14 10:00:00Europe/LondonPumps, Valves, and Fluidic Connectors for Automating and Integrating Microphysiological Systems3D-Printing in the Life Sciences in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Much of the organ-on-chip and tissue-chip research to date has relied on
very simple perfusion schemes that include surface tension, hydrostatic
pressure, rocking plates, syringe pumps, pressurized reservoirs, and
on-chip pneumatic or electromagnetic peristaltic pumps. A number of
commercial microphysiological systems are moving towards high-throughput
well-plate formats. Each of these fluid handling technologies presents
different advantages in cost, convenience, and simplicity, and
disadvantages regarding reservoir filling, media change, recirculation
and volume minimization, organ-organ integration, bubble- and
microbe-free addition and removal of chips from multi-organ
microphysiological systems, temporal control of drug concentrations,
adjusting/balancing flow rates, and controlling sensors for long-term
use. While a common trend is towards high throughput and low cost, there
are critical applications where high content is more important than
high throughput, particularly those requiring multi-omic
characterization of physiological responses over time to drugs and
toxins for both single and multiple organs. We review how our computer
controlled, modular, rotary planar peristaltic micropumps and
twenty-five port rotary planar valves can be used to create
autocalibrating electrochemical MicroClinical Analyzers, multi-well
MicroFormulators that can create in vivo the pharmacokinetics observed
in animals and humans, and perfusion controllers for multiple
NeuroVascular Unit, Gut-in-a Puck, and Thick Tissue Mammary Bioreactors.
Pumps and valves are useful for compact perfusion controllers that
operate in microgravity environments. Miniature rotary valves will
enable bubble-free, sterile connection and disconnection of individual
organ chips to other organs and analyzers. Ongoing and future
applications include 100-port valves and systems for optimizing the
differentiation of induced pluripotent stem cells, a 10,000 channel
yeast MicroChemostat, and a MicroDialysis imager, all with direct
injection into a mass spectrometer. The cost and complexity of these
systems over present technologies is balanced by sophisticated computer
control and sensor integration, reduced technician effort, high content
results, and parallelization.
Add to Calendar ▼2019-10-14 00:00:002019-10-15 00:00:00Europe/London3D-Printing in the Life Sciences3D-Printing in the Life Sciences in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-10-14 09:00:002019-10-14 10:00:00Europe/LondonPumps, Valves, and Fluidic Connectors for Automating and Integrating Microphysiological Systems3D-Printing in the Life Sciences in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
.jpg)
