Ross Jacobson,
Technical Sales Leader,
Particle Metrix, Inc.
Ross Jacobson studied Physics and Chemistry at Hamline University in St. Paul Mn. After receiving his degrees prior to joining Particle Metrix, he performed spectroscopy and diagnostics research for 20+ plus years including 18 years at Becton Dickinsons Corporate Research Center. Throughout his career he has investigated nanoparticles and their applications in diagnostics. He holds six patents in diagnostic applications. Currently he is leading the Western Technical Sales Team for Particle Metrix Inc.
Phenotyping Extracellular Vesicles Using Tetraspanins and Fluorescence-NTA
Monday, 17 February 2020 at 15:00
Add to Calendar ▼2020-02-17 15:00:002020-02-17 16:00:00Europe/LondonPhenotyping Extracellular Vesicles Using Tetraspanins and Fluorescence-NTACirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Nanoparticle Tracking Analysis (NTA) measures size and concentration of nanoparticles in the size range from 10 nm to 1 µm. While getting a particle size distribution, the user typically cannot discriminate whether the particle is a vesicle, protein aggregate, cellular trash or an inorganic precipitate from scatter data alone. The fluorescent detection capability of NTA enables the user to gain biochemical information about the particle and increases the resolution and the discrimination power. NTA instruments traditionally are equipped with one laser wavelength in operation. This limits the detection of biomarkers to only a couple fluorescence dyes. To expand the number of excitation wavelengths, it was necessary to change of the laser source, a time and sample consuming step. To overcome this issue Particle Metrix developed a unique QUATT laser technology and now allows the rapid analysis of biomarker concentrations or ratios with four excitation lasers on the same sample. Here we report the quick and easy EV Phenotyping via multi-wavelength f-NTA on the new Particle Metrix four laser system. Alignment-free switching between excitation wavelengths and measurement mode (scatter and fluorescence) allows quantification of biomarker ratios such as Tetraspanins (CD63, CD81, CD9 and CD41) on the same sample within minutes reducing measurement time and precious sample volumes.
Add to Calendar ▼2020-02-17 00:00:002020-02-18 00:00:00Europe/LondonCirculating Biomarkers World Congress 2020Circulating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2020-02-17 15:00:002020-02-17 16:00:00Europe/LondonPhenotyping Extracellular Vesicles Using Tetraspanins and Fluorescence-NTACirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
