Xiaoli Yu,
Assistant Professor, Department of Neurosurgery,
University of Colorado Anschutz Medical Campus
Dr. Yu received her PhD in Cell and Molecular Biology from Colorado State University. Her postdoc training was in Neuroimmunology focusing on IgG antibodies and related antigens in patients with multiple sclerosis. Currently she is a research faculty in the Department of Neurosurgery at University of Colorado Anschutz Medical Campus. Her projects include the immunopathogenesis of Multiple Sclerosis, focusing on IgG antibodies as biomarkers and antibody-dependent cell-mediated cytotoxicity in demyelination. In addition, she and her collaborators are using phage-displayed random peptide library approaches to identify extracellular vesicles/exosomes as biomarkers for patients with brain tumors, traumatic brain injuries and other CNS disorders.
Applying Phage-Displayed Random Peptide Libraries to Identify Exosomes as Biomarkers in Patients with Central Nervous System Disorders
Tuesday, 18 February 2020 at 08:30
Add to Calendar ▼2020-02-18 08:30:002020-02-18 09:30:00Europe/LondonApplying Phage-Displayed Random Peptide Libraries to Identify Exosomes as Biomarkers in Patients with Central Nervous System DisordersCirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Like most other cells, brain tumor cells shed extracellular vesicles (EV) into their external environment and are considered potentially high-value biomarker reservoirs. We hypothesize that phage-displayed random peptide libraries can be applied to identify EV of CNS origin, and high affinity phage peptides can be used for rapid isolation and characterization of extracellular vesicles from blood of patients with brain tumors. Phage display has been successfully applied to many different areas of research, including immunology, cancer, drug discovery, epitope mapping, protein-protein interactions, and infectious diseases, targeting a broad cross section of protein families. Phage display technology is based on the construction of a polypeptide library fused to a bacteriophage coat protein. Phage are screened (“panned”) on surfaces coated with potential targets. We have applied two Phage-display peptide libraries (7mer &12 mer) and identified high affinity peptides specific to exosomes derived from brain tumor cell-lines and plasma of patients with other CNS disorders. The peptides shared high sequence homologies within the ones selected by the same panning targets as well as with others panned by exosomes from different sources. Our data suggest that phage peptide technologies can be used for enrichment, isolation, and characterization of EVs derived from different CNS cell types from blood of brain tumor patients and other CNS disorders.
Add to Calendar ▼2020-02-17 00:00:002020-02-18 00:00:00Europe/LondonCirculating Biomarkers World Congress 2020Circulating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2020-02-18 08:30:002020-02-18 09:30:00Europe/LondonApplying Phage-Displayed Random Peptide Libraries to Identify Exosomes as Biomarkers in Patients with Central Nervous System DisordersCirculating Biomarkers World Congress 2020 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
