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Complete Next-Generation Gene Expression Workflow for microRNAs from Bodily Fluids, Extracellular Vesicles and Small Sample Input - Sample Preservation, RNA Extraction, Amplification and Detection

Tuesday, 24 March 2015 at 11:00

Add to Calendar ▼2015-03-24 11:00:002015-03-24 12:00:00Europe/LondonComplete Next-Generation Gene Expression Workflow for microRNAs from Bodily Fluids, Extracellular Vesicles and Small Sample Input - Sample Preservation, RNA Extraction, Amplification and DetectionSELECTBIOenquiries@selectbiosciences.com

The tremendous advancements in our basic understanding of cancer biology at the molecular level have led to the increased use of molecules such as DNA, RNA and microRNA (miRNA) as a biomarker for diagnosis and prognosis of various diseases. In particular, much research has focused on detecting biomarkers in bodily fluids such as plasma, serum, urine or saliva, as they are either readily available as clinical samples or they can be easily obtained using non-invasive approaches. The characteristics of RNA present in bodily fluids are quite different from traditional samples such as cells and tissues. First, many of the RNAs in bodily fluids are either free-circulating or are within extra-cellular vesicles such as exosomes. These RNAs are generally small in size - either mRNA of less than 1000 nts or miRNAs. More importantly, the RNA present in bodily fluids is usually of very low abundance and this presents challenges for detection and studies using many next-generation gene expression technologies which require a large amount of RNA template, sometimes at the microgram level.