Shlomit Kenigsberg,
Senior Researcher,
Create Fertility Centre
I am currently a senior researcher and lead for the Ovarian Biology Research group at CReATe Fertility Centre. My research is primarily focused on the isolation and characterizing of exosomes that are secreted from granulosa cells (GLCs) into follicular fluid (FF), and to study their unique RNAs (miRNome) and protein (Proteome) content. We plan to look for differences in exosomes content between different fertility-related patient groups. My long term goals are to 1) provide serum-based biomarkers to help asses a patient’s fertility potential and diagnosis, and 2) to discover potential FF biomarkers to assess egg quality and potential to create a healthy embryo.
Small Volume Biofluid Analysis: Challenges and Solutions for Working with Extracellular and Exosomal RNAs
Monday, 23 March 2015 at 17:00
Add to Calendar ▼2015-03-24 14:00:002015-03-24 15:00:00Europe/LondonSmall Volume Biofluids Analysis: Challenges and Solutions for Extracellular Microvesicles ProfilingSELECTBIOenquiries@selectbiosciences.com
Small volumes of bodily fluids are a vital source for clinical diagnostics, providing important information on tissues and organs. These fluids contain an assortment of biomarkers, including protein complexes, extracellular and vesicular RNAs, each carrying a distinct signature from the point of origin, and potentially cargo for downstream cellular processes. However, many times these samples are limited in volume and precious to get, therefor the analysis of these fluids is inherently challenging. Firstly, these fluids come in extremely small volumes, and thus, only allow for a limited number of tests. Secondly, these fluids contain a large amount of abundant proteins, such as albumin and immunoglobulins and complement factors, which can mask and contaminate the proteomic analyses. Furthermore, extracellular RNAs are not easily separated from the intra-vesicular RNAs and thus, might skew the transcriptome results. Lastly, these fluids can contain a heterogeneous population of extracellular vesicles that are difficult to parse. In this review, I will discuss the sample processing of Cerebrospinal fluid (CSF), synovial fluid, lacrimal fluid, nipple discharge (breast cancer), aspirates from cysts, cervical mucus, uterine cavity aspirates and tubal fluids, with a focus on the challenges we face with our results from follicular and, and embryo culture media, providing some solutions to study these fluids.
Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonExosomes and MicrovesiclesSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2015-03-24 14:00:002015-03-24 15:00:00Europe/LondonSmall Volume Biofluids Analysis: Challenges and Solutions for Extracellular Microvesicles ProfilingSELECTBIOenquiries@selectbiosciences.com
