Hsueh-Chia Chang,
Bayer Professor of Chemical and Biomolecular Engineering,
University of Notre Dame, Interim Chief Technology Officer, Aopia Biosciences
Over 300 publications, with 18,000 citations and h index of 76. He has confounded a startup Aopia Biosciences in 2020 on purification of extracellular vesicles and currently serves as its interim CTO.
A Billion-Droplet AC Electrospray Digital PCR Platform for Large-Dynamic Range Nucleic Acid Quantification
Tuesday, 27 September 2016 at 10:00
Add to Calendar ▼2016-09-27 10:00:002016-09-27 11:00:00Europe/LondonA Billion-Droplet AC Electrospray Digital PCR Platform for Large-Dynamic Range Nucleic Acid QuantificationLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
An AC-electrospray technology is shown to be able to generate 1 billion
femto-liter (micron diameter) aqueous drops in immiscible silicone oil
in less than 20 minutes. Unlike DC sprays, which tend to suffer from
dielectric breakdown in liquid, and droplet generation technologies
based on hydrodynamic shear, which can only generate pico-liter drops,
we have shown that a properly tuned AC field can entrain low-mobility
anions at a meniscus and the Coulombic repulsion among such entrained
ions can deform the meniscus into unique 11 degree AC cones that are
quite distinct from and much stable than DC cones (Phys Rev Lett, 101,
204501(2008); 109, 224301(2012); LabChip, 15, 1656(2015)). The sharper
AC cones can generate 1 million femto-liter drops per second. The
larger drop number and concentration (nM) allow us to get significantly
better accuracy (without using Poisson statistics), dynamic range (8
decades) and reduction of inhibition and interfering effects in
heterogeneous media. We have fabricated an integrated AC electrospray
chip with PCR and imaging modules and have favorably compared the
performance of this new droplet digital PCR platform against qPCR. The
new AC droplet digital PCR platform is, however, a low-cost turn-key
technology that requires no or minimum sample pretreatment.
Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016Lab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-09-27 10:00:002016-09-27 11:00:00Europe/LondonA Billion-Droplet AC Electrospray Digital PCR Platform for Large-Dynamic Range Nucleic Acid QuantificationLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
