Juan Santiago,
Charles Lee Powell Foundation Professor,
Stanford University
Juan G. Santiago received his MS and PhD in Mechanical Engineering from the University of Illinois at Urbana-Champaign in 1995. He holds the Charles Lee Powell Foundation professorship at Stanford and serves as Vice Chair of the Mechanical Engineering Department. His research includes the development of microsystems for on-chip biochemical analysis, microfluidic cell assay systems, two-phase flow devices, and capacitive deionization technologies. Applications of this work include molecular diagnostics, basic studies of cell biology, electronics cooling, and the production of drinking water. He is a Fellow of the American Physical Society, a Fellow of the American Society of Mechanical Engineering, and a Fellow of the American Institute for Medical and Biological Engineering. In 2022, he was elected to the American Academy of Arts and Sciences and to the National Academic of Inventors. He serves as the Editor-in-Chief of the journal Flow. His work is cited about 1500 times per year (Google Scholar h index of 86). He has authored and co-authored over 220 journal papers and 250 conference papers, and he is a named inventor in 58 issued patents (26 of which are currently licensed).
Fractionation and Analysis of Nuclear versus Cytoplasmic Nucleic Acids from Single Cells
Tuesday, 27 September 2016 at 17:35
Add to Calendar ▼2016-09-27 17:35:002016-09-27 18:35:00Europe/LondonFractionation and Analysis of Nuclear versus Cytoplasmic Nucleic Acids from Single CellsLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Single cell analyses (SCA) have become powerful tools in the study
heterogeneous cell populations such as tumors and developing embryos.
However, fractionating and analyzing nuclear versus cytoplasmic
fractions of nucleic acids remains a challenge as these fractions easily
cross-contaminate. We present a novel microfluidic system that can
fractionate and deliver nucleic acid (NA) fractions from the nucleus
(nNA) versus the cytoplasm (cNA) from single cells to independent
downstream analyses. Our technique leverages a selective electrical
lysis which disrupts the cell’s (outer) cytoplasmic membrane, while
leaving the nucleus relatively intact. We selectively extract, purify,
and preconcentrate cNA using isotachophoresis (ITP). The ITP-focused
cNA and nNA-containing nucleus are separated by ITP and fractionated at a
bifurcation downstream and then extracted for off chip analyses. We
will present example applications of this fractionation including qPCR
and next generation sequencing (NGS) analyses of cNA vs. nNA. This will
include preliminary NGS analyses of nuclear vs. cytoplasmic RNA
fractions to analyze gene expression and splicing. We hypothesize that
the robust and precise nature of our electric field control is amenable
to further automation to increase throughput while removing manuals
steps.
Fractionation and Analysis of Nuclear versus Cytoplasmic Nucleic Acids from Single Cells
Tuesday, 27 September 2016 at 17:35
Add to Calendar ▼2016-09-27 17:35:002016-09-27 18:35:00Europe/LondonFractionation and Analysis of Nuclear versus Cytoplasmic Nucleic Acids from Single CellsLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Single cell analyses (SCA) have become powerful tools in the study
heterogeneous cell populations such as tumors and developing embryos.
However, fractionating and analyzing nuclear versus cytoplasmic
fractions of nucleic acids remains a challenge as these fractions easily
cross-contaminate. We present a novel microfluidic system that can
fractionate and deliver nucleic acid (NA) fractions from the nucleus
(nNA) versus the cytoplasm (cNA) from single cells to independent
downstream analyses. Our technique leverages a selective electrical
lysis which disrupts the cell’s (outer) cytoplasmic membrane, while
leaving the nucleus relatively intact. We selectively extract, purify,
and preconcentrate cNA using isotachophoresis (ITP). The ITP-focused
cNA and nNA-containing nucleus are separated by ITP and fractionated at a
bifurcation downstream and then extracted for off chip analyses. We
will present example applications of this fractionation including qPCR
and next generation sequencing (NGS) analyses of cNA vs. nNA. This will
include preliminary NGS analyses of nuclear vs. cytoplasmic RNA
fractions to analyze gene expression and splicing. We hypothesize that
the robust and precise nature of our electric field control is amenable
to further automation to increase throughput while removing manuals
steps.
Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016Lab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-09-27 17:35:002016-09-27 18:35:00Europe/LondonFractionation and Analysis of Nuclear versus Cytoplasmic Nucleic Acids from Single CellsLab-on-a-Chip, Microfluidics and Microarrays World Congress 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
